云南中医药大学中药学院;云南农业大学植物保护学院;云南云天化股份有限公司研发中心;西南林业大学;
[目的]分离、鉴定红大戟根腐病的病原菌及掌握该病原菌的生物学特性。[方法]根据病原菌形态特征,结合ITS-5.8S rDNA、tub2和ACT序列分析,依据柯赫氏法则进行致病性试验。[结果]从接种植株中重新分离出形态一致的病原菌,并将其鉴定为大豆茎点霉(Boeremia exigua)。生物学特性研究表明,病原菌最适生长培养基为PDA培养基,最适生长温度为20℃或25℃,最适培养的pH为5;最适碳源是乳糖,最适氮源是硝酸钾。[结论]这是B.exigua在中国引起红大戟根腐病的首次报道;掌握其生物学特性,为红大戟根腐病的防治奠定理论基础。
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基本信息:
DOI:
中图分类号:S435.672
引用信息:
[1]刘春菊,张磊,李恒等.红大戟根腐病病原鉴定及其生物学特性[J].安徽农业科学,2025,53(02):178-182+186.
基金信息:
云南省重大科技专项计划-生物医药专项(202102AA310037,202102AA310045-04); 云南省科技厅科技计划项目-基础研究计划项目(202001AZ070001-015)