海南大学园艺园林学院;中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地——海南省热带作物栽培生理学重点实验室;中国热带农业科学院橡胶研究所农业部橡胶树生物学重点开放实验室省部共建国家重点实验室培育基地——海南省热带作物栽培生理学重点实验室;
酵母双杂交是利用遗传学方法在酵母真核细胞体内研究蛋白质之间相互作用的非常有效的一种分子生物学技术,被广泛应用于蛋白质组学、细胞信号转导和功能基因组学等领域,已成为分子生物学研究领域的重要实验手段之一,获得了许多有价值的重要发现。随着分子生物学的发展,在酵母双杂交的基础上又建立起了酵母单杂、反向双杂交、三杂交及核外双杂交等多项技术,并已经成功地运用于蛋白质之间、蛋白质与DNA、RNA和配体之间相互作用的研究。对酵母双杂交及其相关技术与应用研究进展进行了综述。可以预见,在人类、水稻和拟南芥等模式生物基因组测序完成后,酵母双杂交及其衍生技术将在功能基因组学和蛋白质组学的研究中发挥越来越重要的作用。
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[1]SOELLICK T R,UHRIG J F.Development of an optimized interaction-matingprotocol for large-scale yeast two-hybrid analyses[J].Genome Biol,2001,2(12):52-60.
[2]FIELDS S,SONG O.A novel genetic system to detect protein-protein interactions[J].Nature,1989,340(6230):245-246.
[3]王关林,方宏筠.植物基因工程[M].2版.北京:科学出版社,2002:242-246.
[4]FIELDS S,STERNGLANZ R.The two-hybrid system:an assay for protein-proteininteractions[J].Trends Genet,1994,10(8):286-292.
[5]YANGM,WU Z,FIELDS S.Protein-peptide interactions analyzed with the yeasttwo-hybrid system[J].Nucleic Acids Res,1995,23(7):1152-1156.
[6]MELEGARI M,SCAGLIONI P P,WANDS J R.Cloning and characterization of anovel hepatitis B virus x binding protein that inhibits viral replication[J].J Vi-rol,1998,72(3):1737-1743.
[7]OKINAKAY,YANG C H,HERMAN E,et al.The P34 syringolide elicitor recep-tor interacts with a soybean photorespiration enzyme,NADH-dependent hydrox-ypyruvate reductase[J].Mol Plant Microbe Interact,2002,15(12):1213-1218.
[8]VRANOV E,T HTIHARJU S,SRIPRANG R,et al.The AKT3 potassiumchannel protein interacts with the AtPP2CA protein phosphatase 2C[J].J ExpBot,2001,52(354):181-182.
[9]BHARTI K,SCHMIDT E,LYCK R,et al.Isolation and characterization of Hs-fA3,a new heat stress transcription factor ofLycopersico nperuvianum[J].PlantJ,2000,22(4):355-365.
[10]SCHOONHEIM P J,VEIGA H,PEREIRA DDA C,et al.A comprehensiveanalysis of the 14-3-3 interactome in barley leaves using a complementary pro-teomics and two-hybrid approach[J].Plant Physiol,2007,143(2):670-683.
[11]FROMONT-RACINE M,RAIN J C,LEGRAIN P.Toward a functional analysisof the yeast genome through exhaustive two-hybrid screens[J].Nat Genet,1997,16(3):277-282.
[12]WALHOUT A J,SORDELLA R,LU X,et al.Protein interaction mapping inC.elegansusing proteins involved in vulval development[J].Science,2000,287(5450):116-122.
[13]SHI ST,POLYAK S J,TUH,et al.Hepatitis C virus NS5A colocalizes with thecore protein on lipid droplets and interacts with apolipoproteins[J].Virol,2002,292(2):198-210.
[14]KISTON J,RAVEN T,JIANG Y P,et al.A death-domain-containing receptorthat mediates apoptosis[J].Nature,1996,384(6607):372-375.
[15]DORTAY H,MEHNERT N,B RKLE L,et al.Analysis of protein interactionswithin the cytokinin-signaling pathway ofArabidopsis thaliana[J].FEBS J,2006,273(20):4631-4644.
[16]BBK.Protein interactions,novel method for identifying protein interactions inproteomics published:(1)[J].Biotech Business Week,2004,22(3):182.
[17]胡承香,徐祥,梁华平,等.酵母双杂交随机肽库的设计及构建[J].动物医学进展,2003,24(2):61-63.
[18]楼鸿飞,张志文.酵母双杂交系统研究进展[J].生物学通报,2001,36(11):1-2.
[19]AUERBACHD,FETCHKOM,STAGLJAR L.Proteomic approaches for generat-ing comprehensive protein interaction maps[J].TARGETS(Drug Discov To-day),2003,2(3):85-92.
[20]LI J J,HERSKOWITZ I.Isolation of ORC6,a component of the yeast originrecognition complex by a one-hybrid system[J].Science,1993,262(5141):1870-1874.
[21]VIDAL M,BRACHMANN R K,FATTAEY A,et al.Reverse two-hybrid andone-hybrid systems to detect dissociation of protein-protein and DNA-proteininteractions[J].Proc Natl Acad Sci USA,1996,93(19):10315-10320.
[22]ARONHEIMA,ZANDI E,HENNEMANNH,et al.Isolation of an AP-1 repres-sor by a novel method for detecting protein-protein interactions[J].Mol CellBiol,1997,17(6):3094-3102.
[23]SENGUPTA D J,ZHANG B,KRAEMER B,et al.A three-hybrid system to de-tect RNA-protein interactions in vivo[J].Proc Natl Acad Sci USA,1996,93(16):8496-8501.
[24]DANG C V,BARRETT J,VILLA-GARCIAM,et al.Intracellular leucine zipperinteractions suggest c-Myc hetero-oligomerization[J].Mol Cell Biol,1991,11(2):954-962.
[25]何康,黄宗道.热带北缘橡胶树栽培[M].广州:广东科技出版社,1987:1-6.
基本信息:
DOI：10.13989/j.cnki.0517-6611.2009.07.040
中图分类号:Q78
引用信息:
[1]李先昆,聂智毅,曾日中.酵母双杂交技术研究与应用进展[J].安徽农业科学,2009,37(07):2867-2869+2926.DOI:10.13989/j.cnki.0517-6611.2009.07.040.
基金信息:
973计划前期研究项目(2007CB116203);; 中国热带农业科学院橡胶研究所基本业务费专项资金项目(2007XJSZX02,XJSYWFZX2008-13)