安徽农业科学

[目的]比较陆地棉和海岛棉种间TCP基因家族成员特征。[方法]利用生物信息学方法对陆地棉和海岛棉TCP基因家族进行全基因组鉴定，并对其理化性质、染色体定位、系统进化和组织表达等方面进行比较分析。[结果]在陆地棉和海岛棉中分别鉴定到69和70个TCP基因，分别具有31对和34对同源基因分布在22和21条染色体上，均为亲水性蛋白。陆地棉和海岛棉之间鉴定到59对同源基因。系统进化关系显示其分为CIN、CYC/TB1和PCF共3个亚族。陆地棉和海岛棉中同亚族的TCP基因家族成员具有相似的基因结构。基因组织表达特异性分析发现，基因Ghir＿D13G006190在陆地棉各组织都有表达，其同源基因Gbar＿D13G006010在海岛棉各组织都没有表达，属于PCF亚类。Ghir＿A09G000900仅在陆地棉花瓣中较高表达，其同源基因Gbar＿A09G000980在海岛棉花瓣、花药、花柱中表达量较高，属于CIN亚类。Ghir＿A12G023760在陆地棉叶中表达量最高，其同源基因Gbar＿A12G023880在海岛棉茎中表达量最高，属于PCF亚类。顺式作用元件分析表明，陆地棉和海岛棉TCP基因家族启动子包含许多与植物光响应、生理响应、激素响应、胁迫响应相关的调控元件。[结论]该研究为进一步探究陆地棉和海岛棉TCP转录因子的功能差异提供重要的理论依据。

[Objective]To compare the characteristics of TCP gene family members between G. hirsutum and G. barbadense.[Method]Bioinformatics methods were used to identify the TCP gene family of G. hirsutum and G. barbadense in the whole genome, and to compare and analyze their physicochemical properties, chromosome localization, systematic evolution and tissue expression.[Result] 69 and 70 TCP genes were identified in G. hirsutum and G. barbadense, respectively, with 31 and 34 pairs of homologous genes distributed on 22 and 21 chromosomes, respectively, all of which were hydrophilic proteins. 59 pairs of homologous genes were identified between G. hirsutum and G. barbadense. The systematic evolutionary relationship showed that it was divided into three subfamilies: CIN, CYC/TB1 and PCF. TCP gene family members of the same subfamily in G. hirsutum and G. barbadense had similar gene structures.Gene tissue-specific expression analysis revealed that the gene Ghir＿D13G006190 was expressed in all tissues of G. hirsutum, while the homologous gene Gbar＿D13G006010 was not expressed in any tissue of G. barbadense, and belonged to the PCF class in the evolutionary tree. Ghir＿A09G000900 was only highly expressed in petals of G. hirsutum, while the homologous gene Gbar＿A09G000980 had the highest expression levels in petals, anthers and styles of G. barbadense, and belongs to the CIN class in the evolutionary tree.Ghir＿A12G023760 was most highly expressed in leaves of G. hirsutum, while the homologous gene Gbar＿A12G023880 was most highly expressed in stems of G. barbadense, and both belong to the PCF class in the evolutionary tree. Cis-acting element analysis showed that the promoters of the TCP gene family of G. hirsutum and G. barbadense contain many regulatory elements related to plant light response, physiological response, hormone response, and stress response. [Conclusion]This study provides an important theoretical basis for further exploring the functional differences of TCP transcription factors between G. hirsutum and G. barbadense.