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[目的]探索冬虫夏草菌合成虫草素的代谢网络，为其深度研究和生物技术应用提供理论依据。[方法]结合高通量测序技术和比较基因组学，完成冬虫夏草菌全基因组测序和组装，预测和鉴定参与虫草素生物合成的潜在关键酶基因。同时，基于液相色谱-质谱联用(LC-MS)的非靶向代谢组学技术，分析冬虫夏草子实体的代谢谱，筛选与虫草素合成相关的差异代谢物。通过关键基因功能与差异代谢物动态的联合分析，并利用KEGG通路富集分析，推断虫草素可能的生物合成途径。[结果]冬虫夏草菌基因组组装结果显示，冬虫夏草菌基因组大小111.4 Mbp, GC含量为45.43%,N50为17.66 Mbp,共注释蛋白编码基因9 519个，平均长度1 481 bp。腺苷激酶、腺苷酸激酶、核糖核酸还原酶、5′-核苷酸酶等可能参与虫草素生物合成。[结论]通过基因组和代谢组整合分析，在系统生物学水平上初步构建了冬虫夏草菌虫草素的生物合成通路网络，鉴定出了多个关键候选基因与代谢节点，为进一步验证功能基因以及利用合成生物学策略生产虫草素奠定了理论基础。
Abstract：[Objective]To investigate the biosynthetic pathway of cordycepin in Ophiocordyceps sinensis, and provide a theoretical basis for in-depth research and biotechnological application[Method]High-throughput sequencing technology was used to sequence and assemble the whole genome of O. sinensis. Potential key enzyme genes involved in cordycepin biosynthesis were predicted and identified through comparative genomic analysis.The metabolic profile of O. sinensis fruiting bodies was analyzed by liquid chromatography-mass spectrometry(LC-MS), identifying differential metabolites associated with cordycepin synthesis. Furthermore, the possible biosynthetic pathway of cordycepin was inferred by integrating key gene functions, dynamic changes of differential metabolites, and KEGG pathway enrichment analysis. [Result] Chromosome-level genome assembly and preliminary analysis revealed that the O. sinensis genome size is 111.4 Mbp with a GC content of 45.43% and an N50 of 17.66 Mbp. A total of 9 519 protein-coding genes were identified, with an average length of 1 481 bp. Enzymes such as adenylate kinase, adenosine kinase, and ribonucleic acid reductase and 5'-nucleotidase were predicted to be involved in cordycepin biosynthesis. [Conclusion] Through genomic and metabolomic integration analysis, the biosynthetic pathway network of cordycepin in O. sinensis was initially constructed at the systems biology level, and multiple key candidate genes and metabolic nodes were identified, laying a theoretical foundation for further verification of functional genes and the production of cordycepin using synthetic biology strategies.
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基本信息:
中图分类号:S567.35
引用信息:
[1]贺元川,王嘉,卫秋阳,等.基于基因组和代谢组学的冬虫夏草菌虫草素生物合成代谢途径研究[J].安徽农业科学,2026,54(08):149-156+186.
基金信息:
重庆市级科研院所绩效激励引导专项(cstc2023jxjl-jbky-X0002); 重庆市技术创新与应用发展专项重点项目(CSTB2024TIAD-KPX0049); 重庆市中药研究院院管项目(zzkjjh2022007); 重庆英才计划创新创业领军人才项目(CQYC20220301627)
2026-04-28
2026-04-28