安徽农业科学

[目的]探讨酸浆组织培养再生植株的最优条件。[方法]以酸浆为材料,以MS培养基为基本培养基,在此基础上添加不同浓度的6-BA和NAA,制成分化培养基,观察不同浓度植物激素对酸浆愈伤组织分化的影响。[结果]当6-BA为低浓度或高浓度时,不定芽总数/材料数和分化率均随NAA浓度的提高而下降;仅当6-BA浓度为1.0 mg/L时,NAA浓度为0.2 mg/L时,不定芽总数/材料数和分化率达到峰值;当NAA浓度一定时,不定芽总数/材料数均在6-BA浓度适中时出现该组的峰值。而分化率呈现不规律变化。[结论]当激素浓度为:1.0 mg/L 6-BA+0.2 mg/L NAA时,酸浆不定芽总数材料数比例和分化率最高。

[Objective] This study aimed to investigate optimal conditions for callus induction and plant regeneration of Physalis alkekengi.[Method] P.alkekengi was employed as the experimental material and different concentrations of 6-BA and α-NAA were added to MS medium to prepare the differentiation medium,to investigate the effect of different concentrations of plant hormones on callus differentiation of P.alkekengi.[Result] Under low or high concentrations of 6-BA,ratio of the total number of adventitious buds/explants and differentiation rate were reduced with the increasing concentration of α-NAA;when the concentrations of 6-BA and α-NAA were respectively 1.0 mg/L and 0.2 mg/L,ratio of the total number of adventitious buds/explants and differentiation rate reached the maximum.When the concentrations of α-NAA were maintained unchanged,ratio of the total number of adventitious buds/explants reached the maximum under the moderate concentrations of 6-BA,while the differentiation rate showed irregular variations.[Conclusion]In MS medium with supplement of 1.0 mg/L of 6-BA and 0.2 mg/L of α-NAA,ratio of the total number of adventitious buds/explants and differentiation rate reached the peak.