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[目的]探究不同光质与6-BA呤对葫芦巴种子萌发、幼苗生长及生理代谢的协同调控效应。[方法]由安徽焦甜香生物科技有限公司提供葫芦巴种子,设置光照条件(自然光、蓝光、红光、红蓝混合光)与6-BA浓度(0、0.5、1.0mg/L)交互处理,测定其种子萌发特性、幼苗形态指标、下胚轴解剖结构及生理指标,通过隶属函数法进行综合评价。[结果]红光条件下施用0.5mg/L 6-BA种子发芽情况、胚芽长度、可溶性蛋白、可溶性糖等指标最高,幼苗丙二醛含量最低;蓝光条件下1.0mg/L 6-BA发芽率、活力指数最低,综合评价最差,但蓝光下类黄酮和花青素含量较高。[结论]红光与0.5mg/L 6-BA组合是提升葫芦巴种子发芽效率、幼苗抗逆性与生长质量的最优处理,红光有利于幼苗下胚轴机械组织发育,而蓝光促进类黄酮和花青素积累。该研究结果为葫芦巴高效育苗提供科学依据与技术支撑。
Abstract：[Objective]To explore the synergistic regulatory effects of different light qualities and 6-benzyladenine(6-BA) concentrations on seed germination,seedling growth,and physiological metabolism of Trigonella foenum-graecum.[Method]Trigonella foenum-graecum seeds were provided by Anhui Jiaotianxiang Biotechnology Co.,Ltd.The seeds were subjected to interactive treatments combining light quality(natural light,blue light,red light,and red-blue mixed light) and 6-BA concentrations(0,0.5,and 1.0 mg/L).Germination characteristics,seedling morphological indicators,hypocotyl anatomical structure,and physiological indices were measured,and a comprehensive evaluation was conducted using the membership function method.[Result]Under red light,application of 0.5 mg/L 6-BA significantly improved seed germination performance,radicle length,soluble protein content,and soluble sugar content,while reducing malondialdehyde(MDA) content—the lowest among all treatments.In contrast,the combination of blue light and 1.0 mg/L 6-BA yielded the lowest germination rate and vigor index,resulting in the poorest comprehensive evaluation score;however,it induced the highest accumulation of flavonoids and anthocyanins under blue light.[Conclusion]The combination of red light and 0.5 mg/L 6-BA is the optimal treatment for enhancing seed germination efficiency,seedling stress resistance,and growth quality of Trigonella foenum-graecum.Red light promotes the development of mechanical tissues in seedling hypocotyls,whereas blue light enhances flavonoid and anthocyanin accumulation.These findings provide a scientific basis and technical support for efficient Trigonella foenum-graecum seedling cultivation.
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基本信息:
中图分类号:S649
引用信息:
[1]徐新,吴璟瑄,杨欣宇,等.不同光质和6-BA对葫芦巴种子萌发及幼苗生长的影响[J].安徽农业科学().
基金信息:
安徽省科技特派团专项(省级科技特派团)(2023tpt066)
2026-09-01
2026-09-01
2026-09-01