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以在温室条件下筛选到的抗病青贮玉米渝青386与感病青贮玉米云瑞121为试验材料，通过多组学联合分析揭示其抗病机制。结果表明：青贮玉米应对小斑病菌侵染时首先通过快速激活初级代谢保障防御所需的ATP和NADPH供给，并启动谷胱甘肽代谢系统缓解病原引发的ROS损伤，维持氧化还原平衡。在次生代谢层面形成核心防御：生物碱合成是关键特异性通路，在抗病品种中持续富集上调，直接抑制病原菌，并与激素介导的信号途径形成快速响应的防御网络；萜类代谢通过合成抗菌化合物并与激素相互调控协同抑菌；苯丙烷代谢持续激活，驱动木质素沉积强化细胞壁物理屏障，并合成紫檀素、二苯庚烷类及黄酮类等抗菌化合物构建化学屏障；信号传导方面MAPK级联通路富集、ABC转运蛋白、植物-病原互作通路在感抗品系中均显著富集。
Abstract：Using the disease-resistant silage corn variety Yuqing 386 and the susceptible variety Yunrui 121 in greenhouse as materials, the disease resistance mechanisms was revealed through multi-omics analysis. The results suggested that in response to Bipolaris maydis infection, silage maize rapidly activates primary metabolism to ensure the supply of ATP and NADPH required for defense. It simultaneously initiates the glutathione metabolism system to mitigate pathogen-induced ROS damage and maintain redox homeostasis.Then, core defense is formed at the level of secondary metabolism: alkaloid biosynthesis is a key specific pathway and it is continuously enriched and upregulated in the resistant variety, directly inhibiting the pathogen and forming a rapidly responsive defense network integrated with hormone-mediated signaling pathways. Terpenoid metabolism synthesizes antimicrobial compounds and synergistically inhibits pathogens through mutual regulation with hormones. Phenylpropanoid metabolism is persistently activated, driving lignin deposition to strengthen the cell wall's physical barrier. It also synthesizes antimicrobial compounds like pterocarpans, diarylheptanoids, and flavonoids to construct a chemical barrier. Signal transduction: pathways including MAPK cascades, ABC transporters, and plant-pathogen interaction were significantly enriched in both resistant and susceptible lines.
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基本信息:
中图分类号:S435.4
引用信息:
[1]刘麒旋,申国境,杨雪飞.基于转录-代谢联合分析挖掘青贮玉米抗小斑病基因[J].安徽农业科学,2026,54(08):76-89.
基金信息:
中国科学院战略性先导科技专项(A类)“放牧性草地补播建植和饲草种质技术示范”(XDA26050301-02)
2025-08-29
2025
2025-09-16
2025-09-30
2025
1
2026-04-28
2026-04-28