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基于小片段文库低深度测序技术对皂荚基因组进行了Survey分析，共获得136.77 Gb数据，经过滤后得到131.14 Gb数据。通过K-mer分析、数据质控、NT比对等步骤，获得皂荚基因组大小780.31 M,重复序列所占比率63.15%,杂合率为0.61%,为微杂合高重复的树种。NT文库比对发现，皂荚比对到近缘物种野皂荚的DNA上，测序文库中不存在明显的外源污染。皂荚基因组特征信息，为进一步开展全基因组染色体水平深度测序，解析药用活性成分的生物合成机制，克隆和挖掘关键功能基因奠定了基础。
Abstract：This study conducted a Survey analysis of the Gleditsia sinensis genome, which based on low-depth sequencing of small fragment libraries, obtaining a total of 136.77 Gb of data. 131.14 Gb of data was obtained after filtering. Through steps such as K-mer analysis, data quality control, and NT alignment, it was obtained that the genome size of Gleditsia sinensis was 780.31 M, the proportion of repetitive sequences was 63.15%, and the heterozygosity rate was 0.61%, which was a tree species with micro-heterozygosity and high repetition. NT library alignment revealed that the DNA of Gleditsia sinensis was aligned with the closely related species Gleditsia microphylla,indicating that there was no obvious exogenous contamination in the sequencing library. This study reports the genomic characteristic information of Gleditsia sinensis, laying a foundation for further conducting deep sequencing at the chromosomal level of the whole genome, analyzing the biosynthesis mechanism of medicinal active components, cloning and mining key functional genes, and optimizing subsequent efficient assembly strategies.
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基本信息:
中图分类号:S792.99
引用信息:
[1]张继妹,陈竹,吉浩,等.皂荚全基因组survey分析[J].安徽农业科学,2026,54(08):95-98.
2025-09-09
2025
2025-09-30
2025-10-14
2025
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2026-04-28
2026-04-28