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[目的]分离筛选具有降胆固醇功能的优良微生物菌株，为开发益生菌菌剂奠定基础。[方法]利用平板划线法从青贮饲料中分离纯化出乳酸菌，利用MRS-CHOL培养基筛选具有高效降胆固醇能力的菌株，并应用环境模拟试验检测其疏水性、自凝集性和交互凝集性，评估菌株对胃肠道环境的耐受性。[结果]分离鉴定了3株副奶酪乳酪杆菌(Lacticaseibacillus paracasei)、1株奶酪乳酪杆菌(Lacticaseibacillus casei)、4株布赫纳氏慢生乳杆菌(Lentilactobacillus buchneri)和1株短发酵剂乳杆菌(Levilactobacillus brevis),L.casei Y3433-2-2-2、L.buchneri Y3434-2-5-1、L.buchneri Y3438-1-7-1、L.buchneri Y3433-1-2-1、L.brevis Y3438-1-77-1、L.buchneri Y3439-2-3-1具有较高的胆固醇清除能力，其降解率分别为52.74%、58.86%、59.62%、58.68%、59.01%、54.47%。胃肠道耐受性试验结果表明，L.casei Y3433-2-2-2、L.buchneri Y3434-2-5-1具有较强胃肠道耐受性，胃液耐受率分别为84.77%、87.36%,肠液耐受率分别为83.52%、85.03%。肠道黏附性试验表明，L.casei Y3433-2-2-2肠道黏附性优于其他菌株；该菌株同时具有较高的抗氧化能力(羟基自由基清除率为92.95%,DPPH自由基清除率为94.45%)、较高的胆盐耐受性(胆盐浓度为0.3%条件下，存活率96.75%);该菌株具有不溶血特性，且对8种病原菌具有抑菌活性。[结论]该研究筛选出一株具有较高的胆固醇清除能力、抗氧化能力、抑菌活性、胃肠道耐受性和不溶血特性的Lacticaseibacillus casei Y3433-2-2-2,为开发降胆固醇益生菌制剂提供科学理论依据。
Abstract：[Objective]The purpose of this study is to isolate and screen excellent microbial strains with cholesterol-lowering capabilities, laying the foundation for the development of probiotic preparations.[Method]Lactic acid bacteria were isolated and purified from silage using the plate streaking method.MRS-CHOL medium was employed to screen strains with high cholesterol-lowering capabilities.Subsequently, environmental simulation tests were conducted to detect their hydrophobicity, auto-aggregation, and co-aggregation abilities, and to assess the tolerance of the strains to the gastrointestinal environment.[Result]In this study, three strains of Lacticaseibacillus paracasei,one strain of Lacticaseibacillus casei,four strains of Lentilactobacillus buchneri,and one strain of Levilactobacillus brevis were isolated and identified.The research found that L.casei Y3433-2-2-2,L.buchneri Y3434-2-5-1,L.buchneri Y3438-1-7-1,L.buchneri Y3433-1-2-1,L.brevis Y3438-1-77-1,and L.buchneri Y3439-2-3-1 exhibited high cholesterol-lowering abilities, with degradation rates of 52.74%,58.86%,59.62%,58.68%,59.01%,and 54.47%,respectively.The results of the gastrointestinal tolerance test showed that L.casei Y3433-2-2-2 and L.buchneri Y3434-2-5-1 had strong gastrointestinal tolerance, with gastric juice tolerance rates of 84.77% and 87.36%,respectively, and intestinal juice tolerance rates of 83.52% and 85.03%,respectively.The intestinal adhesion test indicated that L.casei Y3433-2-2-2 had better intestinal adhesion properties compared to other strains.Additionally, this strain possessed high antioxidant capacity(hydroxyl radical scavenging rate of 92.95%;DPPH radical scavenging rate of 94.45%) and high bile salt tolerance(survival rate of 96.75% at a bile salt concentration of 0.3%).The strain was non-hemolytic and exhibited antibacterial activity against eight bacterial species, including Acinetobacter baumannii,Salmonella enteritidis,Klebsiella pneumoniae,Pseudomonas aeruginosa,Staphylococcus gallinarum,Staphylococcus epidermidis,Enterococcus faecium,and Aeromonas hydrophila.[Conclusion]This study screened a strain of Lacticaseibacillus casei Y3433-2-2-2 with high cholesterol-lowering ability, antioxidant capacity, antibacterial activity, gastrointestinal tolerance, and non-hemolytic properties.This provides a scientific theoretical basis for the development of cholesterol-lowering probiotic preparations.
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基本信息:
中图分类号:TS201.3
引用信息:
[1]王倩,韦燕文,马爱军,等.一株具有降胆固醇功能的奶酪乳酪杆菌的筛选与益生特性研究[J].安徽农业科学,2025,53(24):1-7.
基金信息:
优宜邦生物科技(上海)有限公司产业化项目(UYB20220001-01,UYB(研)20230001-02)