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为获得“十色宝”野生彩茶组培增殖培养最佳方式，以“十色宝”带腋芽的茎段为外植体，采用单因素试验和响应面法研究基础培养基、6-BA、IBA、GA3对“十色宝”野生彩茶继代增殖的影响。结果表明：野生彩茶“十色宝”最佳增殖培养基为B_5+1.07 mg/L 6-BA+0.47 mg/L IBA+2.08 mg/L GA3,增殖率为4.27,该条件下彩茶叶色浓绿，茎干粗壮，重复性好。
Abstract：In order to obtain the best way of tissue culture proliferation of “shisebao” Camellia sinensis, the axillary buds of “shisebao” Camellia sinensis were used as explants. The effects of basic medium, 6-BA, IBA and GA3 on the proliferation of “shisebao” Camellia sinensis were studied by single factor experiment and response surface methodology. The results showed that B_5+1.07 mg/L 6-BA+0.47 mg/L IBA+2.08 mg/L GA3 was the best proliferation medium, and the proliferation rate was 4.27. Under this condition, the stem of “shisebao” Camellia sinensis was thick with dark green leaves, and the repeatability was good.
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基本信息:
中图分类号:S571.1
引用信息:
[1]刘天天,陈雪洋,石战,等.基于响应面法的“十色宝”野生彩茶高效增殖条件筛选与优化[J].安徽农业科学,2025,53(24):32-35.
基金信息:
河南省高等学校重点科研项目计划支持(20B210014)