安徽农业科学

[目的]揭示NaCl胁迫对不同遗传背景玉米幼苗生理生化特性的影响及其适应机制。[方法]以“昭黄20”和“昭黄30”2个自育玉米新品种为研究对象，采用水培方式，探究不同浓度NaCl (0 mmol/L(CK)、25、50、75、100 mmol/L)及不同时间处理对玉米幼苗各项生理生化指标和农艺性状的影响。[结果]不同NaCl胁迫浓度和不同处理时间对不同品种玉米幼苗的生理生化指标和农艺性状的影响不同。盐胁迫能显著抑制玉米幼苗生长发育，并随着胁迫时间和浓度的增加，幼苗生长速度、根长和地上部分的生物量、叶绿素含量和叶片含水量呈减缓或降低趋势。盐胁迫还可导致MDA含量和可溶性蛋白含量增加，细胞内的氧化应激增加。[结论]2个品种均具有一定耐盐性，但遗传背景丰富的新品种“昭黄30号”相较于遗传背景相对单一的“昭黄20”,幼苗耐胁迫性更强。

[Objective]To reveal the effects of NaCl stress on the physiological and biochemical characteristics of maize seedlings with different genetic backgrounds and their adaptation mechanisms.[Method]Two self cultivated maize varieties, “Zhaohuang 20” and “Zhaohuang 30”, were selected as the research objects. Hydroponic cultivation was used to investigate the effects of different concentrations of NaCl(0 mmol/L(CK), 25, 50, 75, 100 mmol/L) and time treatment on various physiological and biochemical indicators and agronomic traits of maize seedlings.[Result]Different concentrations and durations of salt stress have varying effects on the physiological and biochemical indicators as well as agronomic traits of maize seedlings of different varieties. Salt stress can significantly inhibit the growth and development of maize seedlings, and with increasing stress time and concentration, the growth rate, root length, aboveground biomass, chlorophyll content, and leaf water content of seedlings show a slowing or decreasing trend. Salt stress can also lead to an increase in malondialdehyde content and soluble protein content, as well as an increase in intracellular oxidative stress.[Conclusion]Both varieties have certain salt tolerance, but the new variety “Zhaohuang 30” with rich genetic background has stronger seedling stress tolerance compared to the relatively single genetic background “Zhaohuang 20”.