李燕芳,何颖,邹泽红

• 1:广州医学院第二附属医院/呼吸疾病国家重点实验室变态反应研究室/广州市过敏反应与临床免疫重点实验室

摘要(Abstract)：

[目的]预测大米主要过敏原RAG1的二级结构及B细胞表位。[方法]从Expasy蛋白质数据库中获得编码大米过敏原RAG1的氨基酸序列并以此氨基酸序列为基础,通过DNAStar Protean软件,采用Gamier-Robson方案、Chou-Fasman方案和Karplus-Schulz方案预测RAG1的二级结构,用Kyte-Doolittle亲水性方案、Emini表面可及性方案J、ameson-Wolf抗原性指数方案综合预测RAG1的B细胞表位。[结果]对大米过敏原RAG1的二级结构和B细胞表位预测的结果表明,第33～441、19～129、155～163区段可能是B细胞的优势表位。[结论]该研究综合应用多方法多参数预测大米过敏原RAG1潜在的B细胞优势表位,为RAG1 B细胞表位的进一步鉴定及其抗原性改造、表位疫苗设计等研究提供了理论依据。

关键词(KeyWords)： 大米过敏原;RAG1;二级结构;B细胞表位

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30771240);; 广州市教育系统科研创新学术团队(B94118)课题

作者(Author): 李燕芳,何颖,邹泽红

DOI: 10.13989/j.cnki.0517-6611.2012.06.081

参考文献(References)：

• [1]LIM H H,DOMALA Z,JOGINDER S,et al.Rice millers’syndrome:a pre-liminary report[J].British Journal of Industrial Medicine,1984,41(4):445-449.
• [2]Van HOOSER B,CRAWFORD L V.Allergy diets for infants and children[J].Compr Ther,1989,15(10):38-47.
• [3]KIM J H,CHOI GIL-SOON,KIM JEONG-EUN,et al.Three cases of rice-induced occupational asthma[J].Annals of Allergy,Asthma&Immunolo-gy,2010,104(4):353-354.
• [4]KIM J H,KIM J E,CHOI G S,et al.A case of occupational rhinitis causedby rice powder in the grain industry[J].Allergy Asthma Immunol Res,2010,2(2):141-143.
• [5]URISU A,YAMADA K,MASUDA S,et al.16-Kilodalton Rice Protein IsOne of the Major Allergens in Rice Grain Extract and Responsible forCross-Allergenicity between Cereal Grains in the Poaceae Family[J].In-ternational Archives of Allergy and Immunology,1991,96(3):244-252.
• [6]YUICHI T,HIDEHIKO IZUMI,TAKEO KATO,et al.Rice-Seed AllergenicProteins and Hypoallergenic Rice[C]//Nutraceutical Proteins and Pep-tides in Health and Disease.Boca Raton,FL:CRC Press,2005:493-511.
• [7]IZUMI H,ADACHI TAKAHIRO,FUJII NOBORU,et al.Nucleotide se-quence of a cDNA clone encoding a major allergenic protein in rice seedsHomology of the deduced amino acid sequence with members ofα-amyl-ase/trypsin inhibitor family[J].FEBS Letters,1992,302(3):213-216.
• [8]ADACHI T,IZUMI H,YAMADA T,et al.Gene structure and expression ofrice seed allergenic proteins belonging to the alpha-amylase/trypsin inhibi-tor family[J].Plant Mol Biol,1993,21(2):239-248.
• [9]GARNIER J,OSGUTHORPE D J,ROBSON B.Analysis of the accuracyand implications of simple methods for predicting the secondary structureof globular proteins[J].J Mol Biol,1978,120(1):97-120.
• [10]CHOU P,FASMAN G.Prediction of the secondary structure of proteinsfrom their amino acid sequence[J].Adv Enzymol Relat Areas Mol Biol,1978,47:45-148.
• [11]KARPLUS P A,SCHULZ G E.Prediction of chain flexibility in proteins[J].Naturwissenschaften,1985,72(4):212-213.
• [12]KYTE J,DOOLITTLE R.A simple method for displaying the hydropathiccharacter of a protein[J].J Mol Biol,1982.157(1):105-132.
• [13]EMINI E A,HUGHES J V,PERLOW D S,et al.Induction of hepatitis Avirus-neutralizing antibody by a virus-specific synthetic peptide[J].JVirol,1985,55(3):836-839.
• [14]JAMESON B A,WOLF H.The antigenic index:a novel algorithm for pre-dicting antigenic determinants[J].Computer Applications in the Biosci-ences:CABIOS,1988,4(1):181-186.
• [15]吕燕波,万瑛,吴玉章.SARS病毒基因组所编码的E蛋白的二级结构和B细胞表位预测[J].免疫学杂志,2003,19(6):407-410.