徐庆刚;陆健;郑建洲;陈华友;陈克平;

• 1:江苏大学生命科学研究院

摘要(Abstract)：

[目的]对河豚4SNc-Tudor蛋白(SN4TDR)进行序列与结构相关的生物信息分析,为认识该蛋白结构与功能提供借鉴。[方法]应用双向电泳及基质辅助激光解吸离子化-飞行时间质谱(MALD I-TOFMS)技术,从河豚肝脏总蛋白中鉴定到河豚的4SNc-Tudor结构域蛋白。并对其进行生物信息学分析。[结果]首次从河豚肝脏总蛋白中鉴定到河豚的4SNc-Tudor结构域蛋白SN4TDR。河豚SN4TDR定位于细胞质,无信号肽,为非分泌蛋白,有多个磷酸化位点。蛋白SN4TDR存在α-螺旋、β-折叠和无规则卷曲结构,有一个可能形成跨膜结构的片段。并用同源建模的方法,预测了河豚SN4TDR的三维结构模型。[结论]分析河豚SN4TDR基因及蛋白结构,有助于进一步研究该蛋白及其基因的分子和生物学功能。

关键词(KeyWords)： 河豚;MALDI-TOFMS;4SNc-Tudor;生物信息学

Abstract:

Keywords:

基金项目(Foundation): 江苏省高校自然科学基金项目(08KJD180008)

作者(Author): 徐庆刚;陆健;郑建洲;陈华友;陈克平;

Email:

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