马铃薯炭疽病菌分离与鉴定Isolation and Identification of Colletotrichum coccodes
张建成;张慧丽;顾建锋;
摘要(Abstract)：
[目的]对进境船舶上携带的带病马铃薯薯块进行了病原真菌的分离鉴定,明确其检疫重要性,并对病菌的快速鉴定方法进行研究。[方法]通过形态学观察和ITS序列分析对病菌进行了鉴定,并对已有的巢式PCR方法进行了验证。[结果]形态学观察和ITS序列分析表明,病原菌为球炭疽菌(Colletotrichum coccodes)。巢式PCR中用特异性引物Cc1NF1/Cc2NR1扩增C.coccodes得到了349bp特异性条带。[结论]巢式PCR方法可以用来对C.coccodes进行快速鉴定,该病原菌是进境植物检疫潜在危险性病原真菌,在国内口岸属首次截获。
关键词(KeyWords)： 马铃薯炭疽病菌;鉴定;巢式PCR
基金项目(Foundation):
作者(Author): 张建成;张慧丽;顾建锋;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.01.087
参考文献(References)：
- [1]TSROR L,ERLICH O,HAZANOVSKY M.Effect of Colletotrichum coccodes on potato yield,tuber quality,and stem colonization during spring and au-tumn[J].Plant Disease,1999,83(6):561-565.
- [2]姚文国,崔茂森.马铃薯有害生物及其检疫[M].北京:中国农业出版社,2001.
- [3]DILLARD H R.Influence of temperature,pH,osmotic potential,and fungi-cide sensitivity on germination of conidia and growth form sclerotia of Col-letotrichum coccodes in vitro[J].Phytopathology,1988,78:1357-1361.
- [4]JOHNSON D A,MILICZKY E R.Distribution and development of black-dot,Verticillium wilt,and powdery scab on Ruset Burbank potatoes in Washington State[J].Plant Disease,1993,77:74-79.
- [5]易润华,朱西儒,周而勋.简化CTAB法快速微量提取丝状真菌DNA[J].湛江海洋大学学报,2003,23(6):72-73.
- [6]CULLEN D W,LEES A K,TOTH I K,et al.Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR[J].Plant Pathology,2002,51:281-292.
- [7]罗建政,郑建中.船舶食品舱植物检疫方法浅谈[J].植物检疫,2006,20(6):383-384.