马铃薯炭疽病菌分离与鉴定Isolation and Identification of Colletotrichum coccodes
张建成;张慧丽;顾建锋;
摘要(Abstract)：
[目的]对进境船舶上携带的带病马铃薯薯块进行了病原真菌的分离鉴定,明确其检疫重要性,并对病菌的快速鉴定方法进行研究。[方法]通过形态学观察和ITS序列分析对病菌进行了鉴定,并对已有的巢式PCR方法进行了验证。[结果]形态学观察和ITS序列分析表明,病原菌为球炭疽菌(Colletotrichum coccodes)。巢式PCR中用特异性引物Cc1NF1/Cc2NR1扩增C.coccodes得到了349bp特异性条带。[结论]巢式PCR方法可以用来对C.coccodes进行快速鉴定,该病原菌是进境植物检疫潜在危险性病原真菌,在国内口岸属首次截获。
关键词(KeyWords)： 马铃薯炭疽病菌;鉴定;巢式PCR
基金项目(Foundation):
作者(Author): 张建成;张慧丽;顾建锋;
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参考文献(References)：
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