种子特异启动子的克隆及植物表达载体构建Cloning of Seed-specific Promoter and Construction of Plant Transformation Vector
张梦晗;谢伟红;曾洪斌;徐超;李宏业;杨维东;刘洁生;
摘要(Abstract)：
[目的]启动子是植物基因工程中一个重要工具,对构建植物生物反应器有着重要意义。8S球蛋白是绿豆中含量最丰富的种子贮藏蛋白,因此,其调控序列可能提供了一个很好来源的种子特异性启动子。[方法]通过基因组步移技术克隆了绿豆8S球蛋白a亚基基因8SGα的启动子区域,基因组步移使用的引物根据绿豆8SGα的cDNA序列设计。[结果]通过3轮PCR的扩增,得到了472 bp的上游序列片段,构建了植物双元表达载体pBI-8SGα-GUS。[结论]研究结果可用于转化植物,并于转基因植物中分析启动表达的时空特异性及表达强度,并为植物种子生物反应器提供重要元件。
关键词(KeyWords)： 启动子;PCR;基因组步移;绿豆8S球蛋白;种子特异表达载体
基金项目(Foundation): 广东省自然科学基金面上项目(9151063101000001);; 国家大学生创新性实验计划项目(081055908)
作者(Author): 张梦晗;谢伟红;曾洪斌;徐超;李宏业;杨维东;刘洁生;
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