5种桉属植物的miRNA生物信息学预测Bioinformatic Analysis of MicroRNA in 5 Eucalyptus Species
李崇奇;周鹏;蔡望伟;
摘要(Abstract):
[目的]识别桉树miRNA基因和寻找与桉树木质形成相关的miRNA,为进一步开展桉树遗传品质改良工作奠定理论基础。[方法]将mirBase数据库中所有植物的3 228条miRNA序列提交到psRobot网站进行茎环结构预测,应用blast-2.2.27+软件与rfam数据库和pfam数据库比对后去除非miRNA序列,并应用bioedit软件分析miRNA序列及前体序列的碱基组成特点。[结果]共计预测到26条miRNA前体序列和19条不同的成熟miRNA序列,并发现桉树miRNA序列在碱基偏倚现象,5’端第1碱基尿嘧啶出现的频率高达52.6%,而在第19碱基胞嘧啶出现频率为61.1%。靶基因预测发现3个与木质形成相关的miRNA。[结论]应用生物信息学方法首次在桉树中预测到了19条成熟miRNA序列,并识别到了3个与木质形成相关的miRNA。
关键词(KeyWords): 桉树;miRNA;EST
基金项目(Foundation): 海南省研究生创新科研课题(Hyb2012-2)
作者(Author): 李崇奇;周鹏;蔡望伟;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.15.099
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