聂芳;

• 1:襄樊学院

摘要(Abstract)：

[目的]对拟南芥Rac/Rop GEF7(简称GEF7)基因在Rac/Rop GTPse介导的生长素信号途径等方面所起的作用进行初步探索。[方法]利用实验室已构建的稳定表达的拟南芥GEF7基因启动子与GUS融合的转基因植株和GEF7基因过表达的转基因植株,通过GUS组织化学染色分析GEF7基因的表达模式,并对GEF7基因过表达植株的幼苗根系发育情况进行分析。[结果]GEF7基因主要在幼苗根的分生组织和维管组织细胞、侧根原基和根毛细胞表达;经生长素(NAA)诱导处理后,在上述细胞中的表达显著增强。过表达GEF7基因促进侧根的形成。[结论]GEF7基因的功能与根和根毛细胞的发育有关,GEF7基因可能参与对侧根发育的调控。

关键词(KeyWords)： Rac/RopGEF;侧根发生;小G蛋白;生长素

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金“生长素信号转导的分子机理研究”(06025819)

作者(Author): 聂芳;

Email:

DOI: 10.13989/j.cnki.0517-6611.2010.16.037

参考文献(References)：

• [1]TAOLZ,CHEUNGAY,WUHM.Plant Rac-like GTPases are activatedby auxin and mediate auxin-responsive gene expression[J].Plant Cell,2002,14:2745-2760.
• [2]CASIMIRO I,MARCHANT A,BHALERAO R P,et al.Auxin transportpromotesArabidopsislateral root initiation[J].Plant Cell,2001,13:843-852.
• [3]YANGZ.Small GTPases:Versatile Signaling Switches in Plants[J].PlantCell,2002,14(S1):375-388.
• [4]TAO L Z,CHEUNG A Y,CANDIDA N,et al.RAC GTPases in TobaccoandArabidopsisMediate Auxin-Induced Formation of ProteolyticallyActiveNuclear Protein Bodies That Contain AUX/IAA Proteins[J].Plant Cell,2005,17:2369-2383.
• [5]OKUSHIMAY,FUKAKI H,ONODA M,et al.ARF7 and ARF19 regulatelateral rootformationvia directactivationofLBD/ASLgenesinArabidopsis[J].Plant Cell,2007,19:118-130.
• [6]HIMANENK,BOUCHERONE,VANNESTE S,et al.Auxin-mediated cellcycle activation duringearlylateral root initiation[J].Plant Cell,2002,14:2339-2351.
• [7]BENKOVAE,MICHNIEWICZ M,SAUERM,etal.Local,efflux-dependentauxin gradients as a common module for plant organ formation[J].Cell,2003,115:591-602.
• [8]GELDNER N,RICHTER S,VIETEN A,et al.Partial loss-of-function al-leles reveal a role forGNOMin auxin transport-related,post-embryonic de-velopment ofArabidopsis[J].Development,2004,131:389-400.
• [9]LI L,XUJ,XUZ,et al.Brassinosteroids Stimulate Plant Tropisms throughModulation of Polar Auxin Transport in Brassica andArabidopsis[J].PlantCell,2005,17:2738-2753.