小麦液泡膜反转运蛋白基因TaNHX1的生物信息学分析Bioinformatics Analysis of Antiporter Gene TaNHX1 on the Tonoplast Membrane in Wheat
许海霞;李阳;程西永;董中东;李伟;崔党群;
摘要(Abstract)：
[目的]利用生物信息学方法分析基因的功能,[方法]通过生物信息学数据库和因特网上的软件进行分析,对小麦液泡膜Na+/H+反转运蛋白基因TaNHX1的理化性质、结构与功能进行了预测。[结果]TaNHX1基因编码的蛋白是一种相对分子质量为59.7 kD、等电点pI为8.13的疏水性稳定蛋白,富含Leu、Phe、Lle、Gly、Ser、Val、Ala等氨基酸。TaNHX1基因编码的氨基酸序列内含有一段氨氯吡嗪咪的结合域的高度保守序列FF-YLLPI。同源性比较发现TaNHX1与AeNHX1、TiNHX1的亲缘关系很近,分别是99%和97%,推测他们可能为同源基因,具有相似的生物学功能。[结论]该研究为进一步探讨TaNHX1的生物学功能奠定了基础。
关键词(KeyWords)： 小麦;Na+/H+反转运蛋白;TaNHX1基因;生物信息学
基金项目(Foundation): 河南省教育厅自然科学研究项目(2008A208017)
作者(Author): 许海霞;李阳;程西永;董中东;李伟;崔党群;
Email:
DOI: 10.13989/j.cnki.0517-6611.2008.13.068
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