水貂MLPH基因第一外显子序列结构及特征分析Sequence Structure and Characteristics of Exon 1 of Mink MLPH Gene
张敏;
摘要(Abstract):
[目的]克隆辽西地区饲养水貂的MLPH基因第一外显子序列及其侧翼序列,预测其结构特征,为培育彩色水貂提供理论依据。[方法]扩增水貂MLPH基因第一外显子和5'侧翼序列,利用SignalP 3.0 Server、ClustalX 1.83、Mega 4.1、TMHM 2.0、ProtComp-v6.0等生物学软件进行序列分析。[结果]测序得到1 029 bp长的序列片段,利用Mega4.1软件计算水貂与大家鼠的遗传距离是最近的0.540 5,与人的遗传距离是最远的0.655 2,构建水貂分子系统进化树。[结论]水貂MLPH基因是一个分泌蛋白。
关键词(KeyWords): 水貂;MLPH基因;序列结构
基金项目(Foundation): 辽宁医学院博士启动资金资助
作者(Author): 张敏;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.18.226
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