郑浚源;许黎黎;王启要;肖婧凡;

• 1:华东理工大学生物反应器工程国家重点实验室

摘要(Abstract)：

[目的]考察迟钝爱德华氏菌(Edwardsiella tarda)EIB202中大质粒pEIB202在其致病过程中的作用,将质粒pEIB202消除,为开发抗迟钝爱德华氏菌病的安全减毒的活疫苗打下良好基础。[方法]采用同源重组技术以sacB为反向筛选标记消除质粒。[结果]对质粒pEIB202进行序列分析,发现该质粒编码多种抗性基因及部分VI型分泌系统(T4SS)组分,暗示该质粒可能与E.tarda的多重耐药性及致病力相关;质粒消除菌株EIB202Δp丧失氯霉素及四环素抗性,在生长、毒力、胞外蛋白分泌等方面与野生株无显著差异。[结论]PEIB202质粒是造成EIB202多重耐药性的主要原因,在其致病过程中可能并不起直接作用。

关键词(KeyWords)： 迟钝爱德华氏菌(Edwardsiella tarda);pEIB202;T4SS;R质粒;疫苗

Abstract:

Keywords:

基金项目(Foundation): 国家鲆鲽类产业技术体系(CARS-50);; 国家高技术研究计划(863计划)重点项目:迟钝爱德华氏菌毒力相关基因的鉴定及疫苗开发(2008AA092501)

作者(Author): 郑浚源;许黎黎;王启要;肖婧凡;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.29.227

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