李晓丹;张国刚;何庆峰;闫师杰;张平平;

• 1:天津农学院食品科学系
• 2:天津市农副产品深加工技术工程中心
• 3:天津师范大学生命科学学院

摘要(Abstract)：

该研究利用生物信息学软件对克隆得到的油葵基因fad2序列进行核苷酸结构和蛋白质结构分析,结果表明油葵中fad2基因编码382个氨基酸,含有完整的ORF框;其功能域为△-12脂肪酸去饱和酶类结构域,含有3个保守的组氨酸簇;蛋白质二级结构中包含14个α螺旋,7个β折叠,此外还含有17个具有二级结构趋势的无规则卷曲;蛋白质羧基末端以YXXKI结尾,符合Phi-X-X-K/R/D/E-Phi-COOH基序序列规则,是内质网滞留信号;从系统发育进化的角度可知油葵与橄榄的亲缘关系较近。

关键词(KeyWords)： 油葵;fad2;生物信息学;序列分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31100330);; 天津市高等学校科技发展基金计划项目(20100613);; 天津市自然基金(12JCYBJC19700);; 天津师范大学引进人才基金(5RL111)

作者(Author): 李晓丹;张国刚;何庆峰;闫师杰;张平平;

Email:

DOI: 10.13989/j.cnki.0517-6611.2013.06.131

参考文献(References)：

• [1]MOHAN S B,KEKWICK R G.Acetyl-coenzyme A carboxylase from avoca-do(Persea americana)plastids and spinach(Spinacia oleracea)chloroplasts[J].J Biol Chem,1980,187:667-676.
• [2]REITZEL L,NIELSEN N C.Acetyl-CoA carboxylase during development of plastids in wild-type and mutant barley seedlings[J].Eur J Biochem,1976,65:131-138.
• [3]NAGANO Y,MATSUNO R,SASAKI Y,et al.Sequence and transcriptional analysis of the gene cluster trnQ-zpfA-psaI-ORF231-petA in pea chloro-plasts[J].Curr Genet,1991,20:431-436.
• [4]卢善发.植物脂肪酸的生物合成与基因工程[J].植物学通报,2000,17(6):481-491.
• [5]林淑英,宁正祥,郭清泉.共轭亚油酸在食品工业中的应用前景[J].中国油脂,2003,28(11):55-58.
• [6]MARCHLER-BAUER A,BRYANT S H.Protein domain annotations on the fly[J].Nueleie Acids Res,2004,32(13):327-331.
• [7]ZDOBNOV E M,APWEILER R.InterProScan-an integration platform for the signature-recognition methods in InterPro[J].Bioinfonnaties,2001,17:847-848.
• [8]MOCHE M,SHANKLIN J,GHOSHAL A,et al.Azide and acetate comple-xes plus two iron-depleted crystalsetures of the di-iron enzyme delta9 stearoyl-acyl carrier Protein desaturase.ImPlications for oxygen activation and catalytic interrnediates[J].Biol Chem,2003,278(27):25072-25080.
• [9]YANG M,ZHENG G,ZHANG F,et al.FAD2-silencing has pleiotropic effect on polar lipids of leaves and varied effect in different organs of trans-genic tobacco[J].Plant Sci,2006,170(1):170-177.
• [10]PIRTLE I L,KONGCHAROENSUNTORN W,NAMPAISANSUK M,et al.Molecular cloning and functional expression of the gene for a cotton Delta-12fatty acid desaturase(FAD2)[J].Bioehim Biophys,2001,522(2):122-129.
• [11]ADAMS M D,CELNIKER S E,HOLT R A,et al.The genome sequence of Drosophila melanogaster[J].Science,2000,287(5461):2185-2195.
• [12]IWABUCHI M,KOHNO-MURASE J,IMAMURA J.Delta-12-Oleate De-saturase-related Enzymes Associated with Formation of Conjugated trans-Delta11,cis-Delta13Double Bond[J].J Biol Chem,2003,278(7):4603-4610.
• [13]SHANKLIN J,WHITTLE E,FOX B G.Eight histidine residues are cata-lytically essential in a membrane-associated iron enzyme,stearoyl-CoA desaturase,and are conserved in alkane hydroxylase and xylene monooxy-genase[J].Biochemistry,1994,33(43):12787-12794.
• [14]TANHUANPP K,VILKKI J P,VILKKI H J.Mapping and cloning of EAD2gene to develop allele-specific PCR for oleic acid in spring turnip rape(Brassica rapa ssp oleifera)[J].Mol Breed,1995,4:543-550.
• [15]BROADWATER J A,WHITTLE E,SHANKLIN J.Desaturation and hy-droxylation.Residues148and324of Arabidopsis EAD2,in addition to substrate chain length,exert a major influence in Partitioning of catalytic specificity[J].J Biol Chem,2002,277(18):15613-15620.