营养限制对Heynigia riparia CE14-2油脂积累的影响Effects of Nutrient Elements Deficiency on Lipid Accumulation of Heynigia riparia CE14-2
费小雯;蔡佳佳;邓晓东;
摘要(Abstract):
[目的]研究营养限制对Heynigia riparia CE14-2油脂积累的影响。[方法]以从海南热带水域分离得到一藻株为材料,通过18SrDNA同源聚类分析对其进行鉴定。通过将其培养在HSM、SE和BG11 3种系列(缺氮、磷、硫元素的培养基)12种培养基上,检测生长速率和油脂含量。[结果]试验鉴定该藻株为Heynigia riparia CE14-2;Heynigia riparia CE14-2在HSM培养基中生长优于其他培养基,N、P、S缺乏可促使Heynigia riparia CE14-2油脂积累,其中,硫和氮营养缺陷促进油脂积累作用明显,但营养限制使微藻生长减缓,使细胞组分的蛋白质、糖和叶绿素的含量降低;在SE-S培养基中添加葡萄糖或乙酸钠,Heynigia riparia CE14-2的生物量、油脂和叶绿素含量在一定范围内有所增加,但葡萄糖的促进作用不如乙酸钠明显。[结论]为微藻的油脂生产提供了理论依据。
关键词(KeyWords): Heynigia riparia;营养限制;油脂;生长曲线
基金项目(Foundation): 国家自然科学基金项目(30860028,31000117);; 海南省工程技术研究中心专项(GCZX2011006,GCZX2012004)
作者(Author): 费小雯;蔡佳佳;邓晓东;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.34.004
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