费小雯;蔡佳佳;邓晓东;

• 1:海南医学院理学院
• 2:中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室

摘要(Abstract)：

[目的]研究营养限制对Heynigia riparia CE14-2油脂积累的影响。[方法]以从海南热带水域分离得到一藻株为材料,通过18SrDNA同源聚类分析对其进行鉴定。通过将其培养在HSM、SE和BG11 3种系列(缺氮、磷、硫元素的培养基)12种培养基上,检测生长速率和油脂含量。[结果]试验鉴定该藻株为Heynigia riparia CE14-2;Heynigia riparia CE14-2在HSM培养基中生长优于其他培养基,N、P、S缺乏可促使Heynigia riparia CE14-2油脂积累,其中,硫和氮营养缺陷促进油脂积累作用明显,但营养限制使微藻生长减缓,使细胞组分的蛋白质、糖和叶绿素的含量降低;在SE-S培养基中添加葡萄糖或乙酸钠,Heynigia riparia CE14-2的生物量、油脂和叶绿素含量在一定范围内有所增加,但葡萄糖的促进作用不如乙酸钠明显。[结论]为微藻的油脂生产提供了理论依据。

关键词(KeyWords)： Heynigia riparia;营养限制;油脂;生长曲线

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30860028,31000117);; 海南省工程技术研究中心专项(GCZX2011006,GCZX2012004)

作者(Author): 费小雯;蔡佳佳;邓晓东;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.34.004

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