陈亮;陈明霞;李和阳;

• 1:华侨大学化工学院
• 2:国家海洋局第三海洋研究所

摘要(Abstract)：

[目的]对分离自市售高温灭菌牛奶的产脂肪酶菌株SJXYZ进行鉴定。[方法]通过16S rRNA基因序列及系统发育树分析、形态观察和生理生化特性分析,对菌株SJXYZ生物学特性进行初步鉴定。[结果]16S rRNA基因序列分析显示该菌株属于芽胞杆菌属(Bacillus),与B.altitudinis 41KF2b~T(高地芽胞杆菌)、B.xiamenensis HYC-10~T(厦门芽胞杆菌)、B.safensis FO-36b~T(沙福芽胞杆菌)、B.zhangzhouensis DW5-4~T(漳州芽胞杆菌)、B.australimaris NH7I_1~T(南海芽胞杆菌)和B.pumilus A~TCC 7061~T(短小芽胞杆菌)相似度皆为97%以上。系统发育分析显示,该菌株与B.altitudinis 41KF2b~T亲缘关系最近,但形成独立的进化分支,无法归入已知物种。菌株SJXYZ为革兰氏阳性,直杆状,运动性,最适温度为30~35℃,适宜p H为5~9,适宜盐度(Na Cl)为0%~5%;产脂肪酶,不产纤维素酶、淀粉酶、蛋白酶;对氨苄西林(10μg)、庆大霉素(10μg)、氟哌酸(10μg)、头孢唑啉(30μg)、四环素(30μg)、头孢噻吩(30μg)、链霉素(10μg)敏感。所测特性与B.altitudinis 41KF2b~T有较大的差异。[结论]菌株SJXYZ可能为新的物种,暂命名为Bacillus sp.SJXYZ。

关键词(KeyWords)： 芽胞杆菌;菌种鉴定;脂肪酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41506179);; 福建省自然科学基金项目(2015J01613);; “全球变化与海气相互作用”专项(GASI-03-01-03-01,GASI-03-01-03-02);; 华侨大学高层次人才科研启动项目(12BS206)

作者(Author): 陈亮;陈明霞;李和阳;

Email:

DOI: 10.13989/j.cnki.0517-6611.2018.08.031

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