石渠棘球绦虫Es95基因克隆及序列分析Cloning and Sequence Analysis of Es95 Gene from Echinococcus shiquicus
李建秋;李立;闫鸿斌;娄忠子;贾万忠;
摘要(Abstract)：
[目的]首次克隆获得石渠棘球绦虫Es95基因,并进行序列分析,为研究抗包虫病候选疫苗提供理论指导。[方法]从高原鼠兔肺脏包囊上获得石渠棘球绦虫原头蚴,提取全基因组,并根据NCBI上6种棘球属绦虫95基因序列设计引物,以基因组DNA为模板扩增到Es95基因,并克隆到T载体上,测序后进行序列分析。[结果]以石渠棘球绦虫全基因组DNA为模板扩增出Es95基因长为1 330 bp,序列分析含有3个外显子和2个内含子,有1个糖基化位点,N端有一信号肽序列(16个氨基酸),C端有跨膜区(23个氨基酸),亲水性低,B细胞抗原表位预测有ES95潜在抗原表位6个。[结论]ES95是一个分泌性糖蛋白,在包虫病的预防上具有重要意义。
关键词(KeyWords)： 石渠棘球绦虫;ES95蛋白;序列分析
基金项目(Foundation):
作者(Author): 李建秋;李立;闫鸿斌;娄忠子;贾万忠;
Email:
DOI: 10.13989/j.cnki.0517-6611.2015.18.013
参考文献(References)：
- [1]TACKMANN K,MATTIS R,CONRATHS F J.Detection of Echinococcus multilocularis in Foxes:Evaluation of a protocol of the intestinal scraping technique[J].Journal of Veterinary Medicine,Series B,2006,53(8):395-398.
- [2]GUO Z X,HE D L,LI Y Q,et al.Survey of endemic situation and natural foci of hydatidosis in Qinghai-Tibet plateau[J].Int Arch Hydatid,1993,31:69.
- [3]QIU J M,CHEN X W,REN M,et al.Epidemiological study on alveolar hydatid disease in Qinghai-Xizang plateau[J].J Pract Parasit Dis,1995,3:106.
- [4]XIAO N,QIU J M,NAKAO M,et al.Echinococcus shiquicus n.sp.,a taeniid cestode from Tibetan fox and plateau pika in China[J].International Journal for Parasitology,2005,35(6):693-701.
- [5]LIGHTOWLERS M W,LAWRENCE S B,GAUCI C G,et al.Vaccination against hydatidosis using a defined recombinant antigen[J].Parasite Immunology,1996,18(9):457-462.
- [6]HOOP J P,WOOD K R.Prediction of protein antigenic determinants from amino acid sequences[J].Immunology,1981,78(6):3824-3828.
- [7]KARPLUS P A,SCHULZ G E.Prediction of chain flexibiligy in proteins[J].Naturwissenschaften,1985,72(4):212-213.
- [8]JAMESON B A,WOLF H.The antigenic index:A novel algorithm for predicting antigenic determinants[J].Computer Applications in the Biosciences:CABIOS,1988,4:181-186.
- [9]李玉娇,王晶,赵慧,等.细粒棘球绦虫Eg95抗原表位的生物信息学预测[J].中国人兽共患病学报,2011,27(10):892-896.
- [10]范彦雷,李立,娄忠子,等.多房棘球绦虫EM95蛋白的生物信息学分析[J].中国人兽共患病学报,2014,30(4):423-428.