猪繁殖与呼吸综合征病毒非结构蛋白2的生物信息学分析Bioinformatics Analysis of Non-structural Protein 2 of PRRSV
孙荡;高歌;周胜;鲍梦雅;茅翔;
摘要(Abstract):
[目的]为临床上构建PRRSV的拮抗性小肽及相关疫苗设计提供理论参考。[方法]对NCBI上国内分离的PRRSV毒株(FJ175688.1)Nsp2的氨基酸序列进行生物信息学方法分析,并对其组分、理化性质、跨膜结构域、二级结构、糖基化位点、磷酸化位点和B细胞抗原表位进行预测。[结果]Nsp2含有7段的跨膜区域,二级结构中自由卷曲含量最高(57.64%),同时含有4个糖基化位点和76个磷酸化位点。综合分析表明,Nsp2具有大量的抗原决定簇。[结论]通过对Nsp2的生物信息学分析,可为PRRSV疫苗设计提供理论基础。
关键词(KeyWords): PRRSV;非结构蛋白2;生物信息学;结构与功能
基金项目(Foundation): 南京农业大学大学生创新项目(SRT项目)(1004A04)
作者(Author): 孙荡;高歌;周胜;鲍梦雅;茅翔;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.21.011
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