孙荡;高歌;周胜;鲍梦雅;茅翔;

• 1:南京农业大学动物医学院

摘要(Abstract)：

[目的]为临床上构建PRRSV的拮抗性小肽及相关疫苗设计提供理论参考。[方法]对NCBI上国内分离的PRRSV毒株(FJ175688.1)Nsp2的氨基酸序列进行生物信息学方法分析,并对其组分、理化性质、跨膜结构域、二级结构、糖基化位点、磷酸化位点和B细胞抗原表位进行预测。[结果]Nsp2含有7段的跨膜区域,二级结构中自由卷曲含量最高(57.64%),同时含有4个糖基化位点和76个磷酸化位点。综合分析表明,Nsp2具有大量的抗原决定簇。[结论]通过对Nsp2的生物信息学分析,可为PRRSV疫苗设计提供理论基础。

关键词(KeyWords)： PRRSV;非结构蛋白2;生物信息学;结构与功能

Abstract:

Keywords:

基金项目(Foundation): 南京农业大学大学生创新项目(SRT项目)(1004A04)

作者(Author): 孙荡;高歌;周胜;鲍梦雅;茅翔;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.21.011

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