1株感染肉牛的鲍氏不动杆菌的分离与鉴定Isolation and Identification of One Strain of Acinetobacter baumannii in Infected Beef Cattle
李能章;邱荣蓉;牛娟;胡娟;周伟;彭远义;
摘要(Abstract)：
[目的]从疑似患有支原体肺炎肉牛中分离出致病菌,并对其进行鉴定。[方法]从爆发牛支原体肺炎的某牛场病牛肺组织中分离致病菌,并对其进行致病性检测和药敏试验。通过16S rDNA序列测定与系统进化分析,进行病原菌的鉴定。[结果]除分离到牛支原体外,还分离到1株致病性细菌FC-1。该菌革兰氏染色为阴性,球杆状,对大环内酯类药物具有耐药性,对氨基糖苷类及喹诺酮类抗生素敏感。16S rDNA序列测定结果表明该菌为1株鲍氏不动杆菌。[结论]导致牛支原体肺炎的病原除牛支原体外,还可能存在其他病原的混合感染。
关键词(KeyWords)： 支原体肺炎;混合感染;细菌;16SrDNA
基金项目(Foundation): 国家肉牛(牦牛)产业技术体系项目
作者(Author): 李能章;邱荣蓉;牛娟;胡娟;周伟;彭远义;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.05.233
参考文献(References)：
- [1]石磊,龚瑞,尹争艳,等.肉牛传染性牛支原体肺炎流行的诊断.华中农业大学学报,2008,27(5):629-633.
- [2]HALE H H,HELMBOLDT C F,PLASTRIDGE W N,et al.Bovine mastitiscaused by a Mycoplasma species[J].Cornell Vet,1962,52:582-591.
- [3]GOURLAY R N,THOMAS L H,HOWARD C J.Pneumonia and arthritis ingnotobiotic calves following inoculation with Mycoplasma agalactiae subspbovis[J].Vet Rec,1976,98(25):506-507.
- [4]ADEGBOYE D S,HALBUR P G,NUTSCH R G,et al.Mycoplasma bovis-associated pneumonia and arthritis complicated with pyogranulomatous te-nosynovitis in calves[J].J Am Vet Med Assoc,1996,209(3):647-649.
- [5]PFUTZNER H,SACHSE K.Mycoplasma bovis as an agent of mastitis,pneumonia,arthritis and genital disorders in cattle[J].Rev Sci Tech,1996,15(4):1477-1494.
- [6]KIRBY F D,NUCGIKAS R A.Isolation of Mycoplasma bovis from bull-ocks'eyes[J].Vet Rec,1996,138(22):552.
- [7]BOCKLISCH H,KREUSEL S,BRYS A,et al.Experimental infection of theudder of ewes due to Mycoplasma bovis[J].Zentralbl Veterinarmed B,1991,38(5):385-390.
- [8]MAUNSELL F P,DONOVAN G A.Mycoplasma bovis Infections in youngcalves[J].Vet Clin North Am Food Anim Pract,2009,25(1):139-177.
- [9]辛九庆,李媛,郭丹,等.国内首次从患肺炎的犊牛肺脏中分离到牛支原体[J].中国预防兽医学报,2008,30(9):661-664.
- [10]胡长敏,彭清洁,陈颖钰,等.一例奶牛发生牛支原体肺炎的诊断[J].中国奶牛,2011(2):1-3.
- [11]冉智光,谢建华,骆璐,等.我国部分地区牛支原体肺炎和关节炎的病原体诊断[J].中国预防兽医学报,2010(1):40-43.
- [12]尹灿友.西昌某牛场疑似牛支原体肺炎RT-PCR方法的诊断[J].西昌学院学报:自然科学版,2010(1):22-23.