一株新的短芽孢杆菌低温弹性蛋白酶的发酵优化Optimization of Fermentation Conditions for Production of A Extracellular Elastase from A Newly Isolated Bacillus brevis
樊陈;高兆建;张桂英;王东星;
摘要(Abstract)：
[目的]优化短芽孢杆菌XZE116菌株的培养条件使之产生高活性的胞外弹性蛋白酶。[方法]通过单因子和正交试验对XZE116菌株产胞外弹性蛋白酶的培养基及发酵条件进行优化。[结果]该菌株产酶最佳培养基组成为:葡萄糖0.5%、蔗糖0.1%、干酪素0.5%、酵母膏0.5%,K2HPO40.1%,KH2PO40.5%,MgCl20.01%;最佳发酵条件为:250 ml三角烧瓶中装液量40 ml、接种量5%、摇瓶转速200 r/min、pH 8.4、发酵温度为27℃、发酵时间为72 h。XZE116菌株在最佳培养条件下,该菌株的酶活力提高了1.6倍。[结论]研究可为这株新的短芽孢杆菌进行发酵工业生产提供依据,同时为进一步利用该菌株产生的弹性蛋白酶奠定基础。
关键词(KeyWords)： 低温弹性蛋白酶;短芽孢杆菌;发酵优化
基金项目(Foundation): 国家科技富民强县专项行动计划项目(BN2010060);; 江苏省企业博士集聚计划项目;; 大学生创新创业基金项目(201111)
作者(Author): 樊陈;高兆建;张桂英;王东星;
Email:
DOI: 10.13989/j.cnki.0517-6611.2013.22.014
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