基于活的但非可培养细菌降解毒死蜱的研究Research on Viable but Non-culturable Bacteria in Chlorpyrifos Degradation
吴咚咚;王强;蔡慧慧;施洁莹;付惠玲;王新全;周育;丁林贤;
摘要(Abstract)：
[目的]探索降解毒死蜱的新的微生物资源。[方法]应用最大或然数(MPN)法,通过添加适量复苏促进因子(Rpf)从多种土壤及污水生物处理系统中分离活的非可培养(VBNC)资源菌种,根据文献报道的已知毒死蜱降解菌株的系统关系综合分析,初步筛选出与已知降解毒死蜱菌种近缘的VBNC菌株并进行降解试验。[结果]共筛选出6株具有毒死蜱降解能力的菌株,其中菌株CHZYR63对毒死蜱的生物降解能力最强,经Box-behnken响应面法优化后降解率可达70.15%。[结论]可培养化VBNC菌种资源在残留农药降解方面有较大的利用价值,为揭示受染环境中VBNC微生物的形成及其活性化、环境污染物质的共代谢等微生物群体的相互作用机制提供了参考。
关键词(KeyWords)： Rpf;VBNC;毒死蜱;生物降解;响应面法
基金项目(Foundation): 国家高技术研究发展计划(863)项目(2011AA100806);; 国家自然科学基金项目(31340071);; 浙江省自然科学基金项目(LY13C010002)
作者(Author): 吴咚咚;王强;蔡慧慧;施洁莹;付惠玲;王新全;周育;丁林贤;
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参考文献(References)：
- [1]OLIVER J D.Recent findings on the viable but nonculturable state in pathogenic bacteria[J].FEMS Microbiology Reviews,2010,34(4):415-425.
- [2]苏晓梅,丁林贤,沈超峰.活的但非可培养功能菌群对多氯联苯降解的探索进展[J].微生物学报,2013,59(9):908-914.
- [3]MUKAMOLOVA G V,KAPRELYANTS A S,YOUNG D I,et al.A bacterial cytokine[J].Biological Sciences Microbiology,1998,95(15):8916-8921.
- [4]DING L X.Studies on the isolation of viable but nonculturable bacteria and the phylogenetic analysis of the genus Aquaspirillum[D].Tokyo:The University of Tokyo,2004:1-140.
- [5]丁林贤,苏晓梅,横田明.活的但非可培养(VBNC)状态菌的研究进展[J].微生物学报,2011,51(7):858-862.
- [6]SU X M,CHAO F S,SHEN H.A novel approach to stimulate the biphenyldegrading potential of bacterial community from PCBs-contaminated soil of e-waste recycling sites[J].Sci Verse Science Direct,2013,146(13):27-34.
- [7]史延华.毒死蜱降解菌的分离、鉴定及其降解特性[D].北京:中国农业科学院,2011:7-9.
- [8]BAKIAMOH S B,MAIMAIT R,MCGOWIN A E.Supercritical fluid extraction of chlorpyrifos and 3,5,6-trichloro-2-pyridinol from Garden compost[J].Chromatography A,1999,862(1):105-112.
- [9]王金花,陆贻通.农药毒死蜱的生态风险及其微生物修复技术研究进展[J].环境污染与防治,2006,28(2):125-127.
- [10]段海明.毒死蜱降解细菌的筛选、降解特性及其固定化研究[D].泰安:山东农业大学,2011:10-64.
- [11]王川,周巧红,吴振斌.有机磷农药毒死蜱研究进展[J].环境科学与技术,2011,34(7):123-127.
- [12]SEN R,SWAMINATHA T.Response surface modeling and optimization to elucidate and analyze the effects of inoculum age and size on surfactin production[J].Biochemical Engineering Journal,2004,21(2):141-148.
- [13]季宏飞,许杨,李燕萍.采用响应面法优化红曲霉固态发酵产红曲色素培养条件的研究[J].食品科技,2008,27(8):9-13.
- [14]MENG C,CHENG H S,GUO Y H,et al.Study on characteristics of biocometabolic removal of omethoate by the Aspergillus spp.[J].Water Research,2004,38(4):1139-1146.
- [15]MAYA K,SING R S,UPADHYAY S N,et al.Kinetic analysis reveals bacterial efficacy for biodegration of chlorprifors and its hydrolyzing metabolite TCP[J].Process Biochemistry,2011,46(11):2130-2136.
- [16]FENG Y,RACKE K D,BOLLAG J M.Isolation and characterization of a chlorinated-pyridinol-degrading bacterium[J].Applied and Environmental Microbiology,1997,63(10):4096-4098.
- [17]周淑云.毒死蜱高效降解菌的分离鉴定及其生物学特性研究[D].重庆:西南大学,2006:18-57.
- [18]吴祥为,花日茂,操海群,等.毒死蜱降解菌的分离鉴定与降解效能测定[J].环境科学学报,2006,26(9):1433-1439.
- [19]SERAR C M,GIBSON D T,MUNNECKE D M.Plasmid involvement in parathion hydrplysis by Pseudomonas diminuta[J].Appl Environ Microbiol,1982,44(1):246-249.
- [20]陶玉贵,王其进,谷浩,等.哈夫尼菌降解毒死蜱条件的优化及动力学模型建立[J].农业环境科学学报,2011,30(3):449-454.