基于活的但非可培养细菌降解毒死蜱的研究Research on Viable but Non-culturable Bacteria in Chlorpyrifos Degradation
吴咚咚;王强;蔡慧慧;施洁莹;付惠玲;王新全;周育;丁林贤;
摘要(Abstract)：
[目的]探索降解毒死蜱的新的微生物资源。[方法]应用最大或然数(MPN)法,通过添加适量复苏促进因子(Rpf)从多种土壤及污水生物处理系统中分离活的非可培养(VBNC)资源菌种,根据文献报道的已知毒死蜱降解菌株的系统关系综合分析,初步筛选出与已知降解毒死蜱菌种近缘的VBNC菌株并进行降解试验。[结果]共筛选出6株具有毒死蜱降解能力的菌株,其中菌株CHZYR63对毒死蜱的生物降解能力最强,经Box-behnken响应面法优化后降解率可达70.15%。[结论]可培养化VBNC菌种资源在残留农药降解方面有较大的利用价值,为揭示受染环境中VBNC微生物的形成及其活性化、环境污染物质的共代谢等微生物群体的相互作用机制提供了参考。
关键词(KeyWords)： Rpf;VBNC;毒死蜱;生物降解;响应面法
基金项目(Foundation): 国家高技术研究发展计划(863)项目(2011AA100806);; 国家自然科学基金项目(31340071);; 浙江省自然科学基金项目(LY13C010002)
作者(Author): 吴咚咚;王强;蔡慧慧;施洁莹;付惠玲;王新全;周育;丁林贤;
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