田林双;王安琪;陈晏;管娴月;戴传超;

• 1:江苏财经职业技术学院
• 2:南京师范大学生命科学学院

摘要(Abstract)：

[目的]研究花生四烯酸高产突变株的发酵及菌丝老化特性。[方法]对1株经诱变获得的花生四烯酸高产突变株A1.13进行5L罐发酵试验,考察不同培养条件对发酵产物的影响。[结果]碳源一次性过量加入并改变培养温度,可使该菌株花生四烯酸产量提高至602.99 mg/L;发酵菌丝体在4℃条件下老化15 d后,花生四烯酸产量达885.16 mg/L。大豆油、花生油、玉米油、葵花籽油和芝麻油5种植物油均可起到消泡作用,添加浓度4%葵花籽油效果最佳,与对照相比,菌丝生物量和花生四烯酸产量分别提高了124%和22%。[结论]该研究可为突变株A1.13工业化发酵产花生四烯酸提供理论依据和现实参考。

关键词(KeyWords)： 轮梗霉;上罐发酵;植物油;菌丝老化;消泡

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2006BAD27B09);; 江苏省海洋生物技术重点建设实验室开放项目(2007HS012)

作者(Author): 田林双;王安琪;陈晏;管娴月;戴传超;

Email:

DOI: 10.13989/j.cnki.0517-6611.2009.35.026

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