花生四烯酸高产突变株5L罐发酵及菌丝老化研究Study on Fermentation of High Arachidonic Acid-producing Mutant in 5 L Fermenter and Mycelium Aging
田林双;王安琪;陈晏;管娴月;戴传超;
摘要(Abstract):
[目的]研究花生四烯酸高产突变株的发酵及菌丝老化特性。[方法]对1株经诱变获得的花生四烯酸高产突变株A1.13进行5L罐发酵试验,考察不同培养条件对发酵产物的影响。[结果]碳源一次性过量加入并改变培养温度,可使该菌株花生四烯酸产量提高至602.99 mg/L;发酵菌丝体在4℃条件下老化15 d后,花生四烯酸产量达885.16 mg/L。大豆油、花生油、玉米油、葵花籽油和芝麻油5种植物油均可起到消泡作用,添加浓度4%葵花籽油效果最佳,与对照相比,菌丝生物量和花生四烯酸产量分别提高了124%和22%。[结论]该研究可为突变株A1.13工业化发酵产花生四烯酸提供理论依据和现实参考。
关键词(KeyWords): 轮梗霉;上罐发酵;植物油;菌丝老化;消泡
基金项目(Foundation): 国家科技支撑计划项目(2006BAD27B09);; 江苏省海洋生物技术重点建设实验室开放项目(2007HS012)
作者(Author): 田林双;王安琪;陈晏;管娴月;戴传超;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.35.026
参考文献(References):
- [1]JAMESG METZ,PAUL ROESSLER,DANIEL FACCIOTTI,et al.Produc-tion of polyunsaturated fatty acids by polyketide synthases in both pro-karyotes and eukaryotes[J].Science,2001,293:290-293.
- [2]EIJI SAKURADANI,TAKAHIROABE,KEITAIGUCHI,etal.Anovel fun-galω3-desaturase withwide substrate specificityfromarachidonic acid-pro-ducingMortierella alpina1S-4[J].Appl Microbiol Biotechnol,2005,66:648-654.
- [3]刘吉华,袁生,戴传超.二十碳五烯酸等多不饱和脂肪酸的筛选[J].菌物系统,2000,19(3):407-409.
- [4]王安琪,戴传超,赵沫,等.轮梗霉高产花生四烯酸的诱变育种研究[J].食品科学,2007,28(2):158-162.
- [5]朱法科,鲍时翔,林炜铁,等.菌丝体老化对被孢霉菌产花生四烯酸的影响[J].食品科学,1997,22(3):40-42.
- [6]MINGHC,TERRYHW,GREGORYJ H,etal.Fungal production ofeico-sapentaenoic and arachidonic acids from in dustrial waste streams andcrude soybean oil[J].Bioresource Technology,1999,67:101-110.
- [7]SANDRAD DYAL,SURESHS NARINE.Implications for the use of Mor-tierella alpine fungi in the industrial production ofessential fattyacids[J].Food Research International,2005,38:445-467
- [8]肖瀛,李柏林,欧杰,等.深黄被孢霉在不同培养条件下合成GLA和代谢副产物脂肪酸的分析[J].食品科学,2007,28(3):241-246.
- [9]SAJBIDOR J.Effect of some environmental factors on the contentand com-position of microbial membrane lipids[J].Crit Rev Biotechnol,1997,17:87-103.
- [10]SINGHA,WILSONS.Docosahexaenoic acid productionbyThraustochytri-umsp.ATCC20892[J].World J Microbiol Biotech,1996,12:76-81.
- [11]ZHULY,ZHANGX C,SONG X J,et al.Effect of temperature,salinity,pH,carbon and nitrogen sources on the growth and docosahexaenoic acidproduction ofSchizochytrium limacinum[J].Process Biochem,2007,42(2):210-214.
- [12]SHINMENY,SHIMIZUS.Production of arachidonic acid byMortierellaelogataIS-4[J].Appl Microbiol Biotechnol,1989,91:11-16.
- [13]BAJPAI P,BAJPAI P K,WARD OP.Effects of agingMortierellamyceli-umon production of arachidonic and eicosapentaenoic acids[J].JAOCS,1991,68(10):775-780.