张建伟;史爱华;沈佳;景小冬;章振华;李林;姜北宇;

• 1:北京市农林科学院畜牧兽医研究所

摘要(Abstract)：

[目的]比较3种培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果。[方法]采用DMEM+10%血清、低血清培养基(MEM-MD-611)、无血清培养基(SFE4Mega)3种培养基制备MDCK细胞单层,分别接种不同稀释度的H9亚型禽流感病毒。然后,分别加入含10μg/ml胰蛋白酶的3种培养基作为维持液,培养病毒,每隔24 h观察其细胞病变,并测定上清HA滴度。[结果]在培养72～96 h时,无血清培养基病毒液的HA滴度高于低血清培养基,而低血清培养基则高于含血清培养基。[结论]3种培养基均可用于制备禽流感病毒抗原,其中无血清培养基、低血清培养基更适用于流感病毒抗原的制备。

关键词(KeyWords)： 培养基;H9亚型流感病毒;MDCK细胞;增殖效果

Abstract:

Keywords:

基金项目(Foundation): 北京市农林科学院一般项目(2010A007)

作者(Author): 张建伟;史爱华;沈佳;景小冬;章振华;李林;姜北宇;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.08.010

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