阳永学;程维舜;曾红霞;张娜;施先锋;孙玉宏;

• 1:武汉市农业科学研究所

摘要(Abstract)：

[目的]克隆西瓜半胱氨酸蛋白酶基因ClCP1,并对其进行生物信息学分析。[方法]以西瓜花蕾cDNA为模板,借助西瓜全基因组数据库,采用RT-PCR方法对西瓜半胱氨酸蛋白酶基因进行克隆并进行生物信息学分析。[结果]克隆得到一个开放阅读框长度为960 bp的序列,命名为ClCP1。该基因编码319个氨基酸。ClCP1基因的保守结构域分析结果表明,ClCP1属于木瓜蛋白酶家族基因,与已知其他植物半胱氨酸蛋白酶在氨基酸序列上有56%~85%的相似性。聚类分析结果显示,ClCP1与同为葫芦科的黄瓜CP基因亲缘关系较近。[结论]ClCP1属于西瓜木瓜蛋白酶家族新基因,为进一步研究西瓜半胱氨酸蛋白酶基因ClCP1的表达和功能奠定了基础。

关键词(KeyWords)： 西瓜;ClCP1;基因克隆;生物信息学

Abstract:

Keywords:

基金项目(Foundation): 武汉市农业科学技术研究院创新项目(CX201242)

作者(Author): 阳永学;程维舜;曾红霞;张娜;施先锋;孙玉宏;

Email:

DOI:

参考文献(References)：

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