带有组氨酸标签的蛇毒基因在毕赤酵母中的表达Expression of A Snake Venom Gene with Histidine Tag in Pichia pastoris
黎卓键;
摘要(Abstract):
[目的]通过巴斯德毕赤酵母(Pichia pastoris)表达带有组氨酸标签的蛇毒基因。[方法]利用基因重组技术,以海蛇基因为材料,通过PCR技术在N末端添加一个六聚组氨酸纯化标签,并将重组质粒导入菌株GS115,用1%甲醇诱导后,分泌表达了重组蛋白。[结果]测序结果显示该标签已成功插入,SDS-PAGE检测到分子量为28.5 kD的目的蛋白。[结论]成功表达了带有组氨酸标签的蛇毒蛋白,其具有良好的降纤活性。
关键词(KeyWords): 蛇毒基因;毕赤酵母;分泌表达
基金项目(Foundation): 广东省教育部产学研结合项目(2009B090300284)
作者(Author): 黎卓键;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.31.008
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