丁懿;石彩娟;王万军;

• 1:西南交通大学生命科学与工程学院

摘要(Abstract)：

作为植物中最重要的生长素外输载体,PIN家族在各种植物中都拥有众多成员。对水稻基因组的BLAST搜索获得了12个PIN家族成员,分析发现:水稻PIN基因不均衡地分布在基因组染色体上;其内含子、外显子结构类似;PIN蛋白质拥有典型的载体蛋白序列特征,即亲/疏水性反复变化,存在多次跨膜结构域;几乎所有PIN蛋白都有一个NPNXY的内化结构域和7个功能未知的基序;PIN在早期即分化为两组成员,主要区别在于中间亲水环的部分缺失;并且在后来发生了支系特异的复制事件,从而形成了众多旁系同源基因。

关键词(KeyWords)： PIN家族;水稻;生物信息学

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 丁懿;石彩娟;王万军;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.27.118

参考文献(References)：

• [1]WALLROTH-MARMONR L,HARTE C.IAA in leaves of Antirhinum majusL.Sippe 50 and some mutants[J].Biologisches Zentralblatt,1988,197:517-531.
• [2]SCHERES B,BERLETH T.Root development:new meanings for root ca-nals?[J]Curr Opin Plant Biol,1998,1:32-36.
• [3]NEWCOMB W,WETHERELL D F.The effects of 2,4,6-trichlorophenoxy-acetic acid on embryogenesis in wild type carrot tissue cultures[J].BotGaz,1970,131:242-245.
• [4]FRY S C,WANGERMANN E.Polar transport of auxin through embryos[J].New Phytol,1976,77:313-317.
• [5]SCHIAVONE F M,COOKE T J.Unusual patterns of somatic embryogenesisin the domesticated carrot:developmental effects of exogenous auxins andauxin transport inhibitors[J].Cell Diff,1987,21:53-62.
• [6]OKADA K,UEADA J,KOMAKI M K,et al.Requirement of the auxin polartransport system in early stages of Arabidopsis floral bud formation[J].Plant Cell,1991,3:677-684.
• [7]GLWEILER L,GUAN C,MLLER A,et al.Regulation of polar auxintransport by AtPIN1 in Arabidopsis vascular tissue[J].Science,1998,282:2226-2230.
• [8]PALME K,GLWEILER L.PIN-pointing the molecular basis of auxintransport[J].Current Opinion in Plant Biology,1999,2:375-381.
• [9]PAVEL KRˇE CˇEK,PETR SKUPA,JIRˇLIBUS,et al.The PIN-FORMED(PIN)protein family of auxin transporters[J].Genome Biology,2009,10:249.
• [10]PAPONOV I A,TEALE W D,TREBAR M,et al.The PIN auxin efflux fa-cilitators:evolutionary and functional perspectives[J].Trends in PlantScience,2005,10(4):170-177.
• [11]王继荣.水稻PIN家族基因表达模式研究[D].杭州:浙江大学,2008.
• [12]ALTSCHUL S F,GISH W,MILLER W,et al.Basic local alignment searchtool[J].J Mol Biol,1990,215:403-410.
• [13]GUO A Y,ZHU Q H,CHEN X,et al.GSDS:a gene structure display serv-er[J].Yi Chuan,2007,29(8):1023-1026.
• [14]EMANUELSSON O,BRUNAK S,von HEIJNE G,et al.Locating proteinsin the cell using TargetP,SignalP and related tools[J].Nature Protocols,2007,2(4):953-971.
• [15]IKEDA M,ARAI M,LAO D M,et al.Transmembrane topology predictionmethods:a reassessment and improvement by a consensus method using adataset of experimentally characterized transmembrane topologies[J].InSilico Bio,2002,2(1):19-33.
• [16]HALL T A.BioEdit:a user-friendly biological sequence alignment editorand analysis program for Windows 95/98/NT[J].Nucl Acids Symp Ser,1999,41:95-98.
• [17]BAILEY T L,ELKAN C.Fitting a mixture model by expectation maximi-zation to discover motifs in biopolymers[C]//Proceedings of the SecondInternational Conference on Intelligent Systems for Molecular Biology.Menlo Park,California:AAAI Press,1994:28-36.
• [18]LARKIN M A,BLACKSHIELDS G,BROWN N P,et al.Clustal W andClustal X version 2.0[J].Bioinformatics,2007,23:2947-2948.
• [19]KUMAR S,NEI M,DUDLEY J,et al.MEGA:a biologist centric softwarefor evolutionary analysis of DNA and protein sequences[J].Brief Bioin-form,2008,9(4):299-306.
• [20]PATERSON A H,BOWERS J E,PETERSON D G,et al.Structure and e-volution of cereal genomes[J].Current Opinion in Genetics and Develop-ment,2003,13:644-650.
• [21]PATERSON A H,BOWERS J E,CHAPMAN B A.Ancient polyploidiza-tion predating divergence of the cereals,and its consequences for compar-ative genomics[J].Proceedings of the National Academy of Sciences,2004,101:9903-9908.
• [22]WANG X Y,SHI X L,HAO B L,et al.Duplication and DNA segmentalloss in the rice genome:implications for diploidization[J].New Phytolo-gist,2005,165:937-946.
• [23]GASTEIGER E,HOOGLAND C,GATTIKER A,et al.Protein identifica-tion and analysis tools on the ExPASy server[M]//WALKER J M.TheProteomics Protocols Handbook.New Jersey:Humana Press,2005:571-607.
• [24]于胜楠,崔继哲.PIN蛋白在生长素极性运输中的作用[J].生物技术通报,2009(3):20-24.
• [25]FORCE A,LYNCH M,PICKETT F B,et al.Preservation of duplicategenes by complementary,degenerative mutations[J].Genetics,1999,151:1531-1545.