人参根际土壤微生物PCR-DGGE反应体系优化Optimization of PCR-DGGE Reaction System on Rhizosphere Soil Microorganism for Panax ginseng C.A.Mey.
刘权钢;金海林;唐丽娜;金东淳;
摘要(Abstract):
[目的]优选人参根际土壤微生物的PCR-DGGE反应体系。[方法]以种植1年的人参土壤为材料,利用PCR-DGGE技术,对反应体系中的几种重要参数不同梯度进行了优化研究,包括模板、dNTPs、引物及Mg2+的用量。[结果]最适宜的反应体系为:模板DNA浓度为0.8μg/μl,dNTPs浓度为0.2 mmol/L,引物浓度为0.4μmol/L,Mg2+浓度为1.5 mmol/L。[结论]该方法简便快捷,为进一步研究人参根际土壤微生物多样性奠定了基础。
关键词(KeyWords): 人参(Panax ginseng C.A.Mey.);根际土壤微生物;PCR-DGGE;体系优化
基金项目(Foundation):
作者(Author): 刘权钢;金海林;唐丽娜;金东淳;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.12.189
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