日本乙型脑炎病毒分离株E基因的克隆及序列分析Cloning and Sequence Analysis of E Genes of Japanese Encephalitis Virus Isolated Strain
陈梅;王艳允;
摘要(Abstract):
[目的]对日本乙型脑炎病毒分离株E基因进行克隆及序列分析。[方法]根据乙型脑炎病毒SA14和SA14-14-2株序列,设计并合成一对E基因的特异性引物,用RT-PCR方法扩增出JEV分离株E基因片段,将其与pMD19-T载体连接,应用DNAStar、Clustal_1.81和Mega序列分析软件进行同源性比较。[结果]分离株E基因的cDNA长1 500 bp,可编码500个氨基酸;分离株E基因与VN50/Viet Nam/1989/Hu-man brain株、SA14株、Whe株、Benjing 1株、P3株、KV1889株J、aGAr01株和SA14-14-2株的核苷酸同源性为88.0%~99.1%,氨基酸同源性为97.8%~99.8%;分离株为乙型脑炎病毒强毒株。[结论]该研究为乙脑病毒基因工程疫苗的研发奠定了一定的基础。
关键词(KeyWords): 乙型脑炎病毒;分离株;E基因;克隆;序列分析
基金项目(Foundation):
作者(Author): 陈梅;王艳允;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.13.011
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