麻疯树cpSSR标记技术的建立与体系优化研究Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn
向倩;周兰英;万静;张旭;雷宝盛;金银春;冯毅;赵晓英;于绪任;
摘要(Abstract):
[目的]建立麻疯树cpSSR-PCR反应的最佳反应体系。[方法]对影响麻疯树cpSSR-PCR反应体系的5个因素(TaqDNA聚合酶、DNA模板、Mg2+、dNTP、引物)进行优化试验,筛选各反应因素的最佳水平。[结果]20μl反应体系各组分的最合适浓度分别为10×Buffer、2.00mmol/LMg2+、2U/μlTaqDNA聚合酶、0.2mmol/LdNTP、0.2μmol/L引物、35ng/μlDNA模板。[结论]最佳的退火温度为52℃;该反应体系的稳定性和可重复性均较好。
关键词(KeyWords): 麻疯树;cpSSR-PCR;体系优化
基金项目(Foundation):
作者(Author): 向倩;周兰英;万静;张旭;雷宝盛;金银春;冯毅;赵晓英;于绪任;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.32.075
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