李金磊;董鹏;狄元冉;方忠意;张发旺;周红霞;吴志明;高延玲;

• 1:河南省兽药饲料监察所

摘要(Abstract)：

[目的]了解饲料及饲料原料中致泻性大肠杆菌的污染情况。[方法]2015—2016年,应用多重PCR技术对463批饲料及饲料原料中致泻性大肠杆菌进行检测。[结果]463批样品共检出12批阳性样品,检出率为2.6%,其中以EPEC、ETEC、EHEC为主。EPEC阳性样品7批,阳性率1.50%;ETEC阳性样品3批,阳性率0.65%;EHEC阳性样品2批,阳性率0.43%。[结论]饲料和饲料原料中致泻性大肠杆菌污染比较严重,应进一步加强管理。

关键词(KeyWords)： 饲料;饲料原料;致泻性大肠杆菌;多重PCR;检测

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李金磊;董鹏;狄元冉;方忠意;张发旺;周红霞;吴志明;高延玲;

Email:

DOI: 10.13989/j.cnki.0517-6611.2018.03.047

参考文献(References)：

• [1]LIU L,JOHNSON H L,COUSENS S,et al.Child health epidemiology reference group of WHO and UNICEF:Global,regional,and national causes of child mortality:An updated systematic analysis for 2010 with time trends since 2000[J].Lancet,2012,379:2151-2161.
• [2]KOTLOFF K L,NATARO J P,BLACKWELDER W C,et al.Burden and aetiology of diarrhoeal disease in infants and young children in developing countries(the global enteric multicenter study,GEMS):A prospective,case-control study[J].Lancet,2013,382(9888):209-222.
• [3]ASSIS F E A,WOLF S,SUREK M,et al.Impact of Aeromonas and diarrheagenic Escherichia coli screening in patients with diarrhea in Paraná,southern Brazil[J].J Infect Dev Ctries,2014,8(12):1609-1614.
• [4]DUTTA S,PAZHANI G P,NATARO J P,et al.Heterogenic virulence in a diarrheagenic Escherichia coli:Evidence for an EPEC expressing heat-labile toxin of ETEC[J].International journal of medical microbiology,2014,305(1):47-54.
• [5]CROXEN M A,LAW R J,SCHOLZ R,et al.Recent advances in understanding enteric pathogenic Escherichia coli[J].Clin Microbiol Rev,2013,26(4):822-880.
• [6]NATARO J P,KAPER J B.Diarrheagenic Escherichia coli[J].Clin Microbiol Rev,1998,11(1):142-201.
• [7]CAINE L A,NWODO U U,OKOH A I,et al.Occurrence of virulence genes associated with diarrheagenic Escherichia coli isolated from raw cow's milk from two commercial dairy farms in the eastern cape province,South Africa[J].Int J Environ Res Public Health,2014,11:11950-11963.
• [8]GMEZ-DUARTE O G,ARZUZA O,URBINA D,et al.Detection of Escherichia coli enteropathogens by multiplex polymerase chain reaction from children's diarrheal stools in two Caribbean-Colombian cities[J].Foodborne Pathog Dis,2010,7(2):199-206.
• [9]RAJENDRAN P,AJJAMPUR S S R,CHIDAMBARAM D,et al.Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India[J].Diagn Microbiol Infect Dis,2010,68(2):117-122.
• [10]OPINTAN J A,BISHAR R A,NEWMAN M J,et al.Carriage of diarrhoeagenic Escherichia coli by older children and adults in Accra,Ghana[J].Transactions of the royal society of tropical medicine and hygiene,2010,104:504-506.
• [11]FRANK C,WERBER D,CRAMER J P,et al.Epidemic profile of Shigatoxin-producing Escherichia coli O104:H4 outbreak in Germany[J].N Engl J Med,2011,365(19):1771-1780.
• [12]朱林英,赵冰,黄红,等.分子诊断技术结合传统分离方法在致泻性大肠杆菌检测中的运用[J].中国卫生检验杂志,2015,25(4):467-470.
• [13]ESTRADA-GARCIA T,NAVARRO-GARCIA F.Enteroaggregative Escherichia coli pathotype:A genetically heterogeneous emerging foodborne enteropathogen[J].FEMS Immunol Med Microbiol,2012,66(3):281-298.
• [14]PATZI-VARGAS S,ZAIDI M B,PEREZ-MARTINEZ I,et al.Diarrheagenic Escherichia coli carrying supplementary virulence genes are an important cause of moderate to severe diarrhoeal disease in mexico[J].PLo S Negl Trop Dis,2015,9(3):1-18.
• [15]JANDA J M,ABBOTT S L.Revisiting bacterial gastroenteritis,Part I:Issues,possible approaches,and an ever-expanding list of etiologic agents[J].Clin Microbiol Newsletter,2010,33(10):