刘涛;许颖妍;熊青;赵金星;陈建军;陈桂信;佘文琴;

• 1:福建农林大学园艺学院园艺产品贮藏保鲜研究所

摘要(Abstract)：

[目的]克隆夜香树(Cestrum nocturnumL.)萜类香气物质代谢途径关键限速酶DXS2(1-deoxy-D-xylulose-5-phosphate synthase 2)基因全长cDNA,并对该基因进行生物信息学分析,检测该基因在开花不同时间及叶片中表达量的动态变化。[方法]采用RTPCR与RACE相结合的技术,获得夜香树DXS2的全长核苷酸序列,并进行生物信息学分析,qRT-PCR分析其在盛花期不同时间花和叶中的表达量。[结果]获得CnDXS2基因全长序列2 370 bp,其中ORF 2 145 bp,编码714个氨基酸,含DXP-synthase-N、TPP-PYRDXS-TK-like和Transketolase-C等保守结构域,与绒毛状烟草(Nicotiana tomentosiformis L.)DXS2蛋白同源性达93%,推测属于Ⅱ类DXS蛋白;qRT-PCR分析表明,CnDXS2在叶中表达量总体高于花,花中22∶00表达量最高,14∶00表达量最低,具有节律性。[结论]萜类MEP代谢途径基因CnDXS2的克隆与表达分析为从分子方面揭示CnDXS2基因在萜类香气代谢途径中的功能提供了依据,有助于夜香树花香萜类代谢途径基因功能和花香基因工程的研究。

关键词(KeyWords)： 夜香树;DXS2;表达分析

Abstract:

Keywords:

基金项目(Foundation): 福建自然科学基金项目(闽财指[2013]891号)

作者(Author): 刘涛;许颖妍;熊青;赵金星;陈建军;陈桂信;佘文琴;

Email:

DOI: 10.13989/j.cnki.0517-6611.2017.10.045

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