陈亮;崔堂兵;

• 1:华南理工大学生物科学与工程学院

摘要(Abstract)：

[目的]研究豆豉纤维酶(DFE)在枯草杆菌WB800中的生理功能。[方法]以能够合成DFE的重组菌株WB800/pBE3.DFE为试验菌株,研究其产孢率和在各外界胁迫下的耐受性。[结果]菌株WB800/pBE3和WB800/pBE3.DFE在产孢培养基中发酵24 h后,菌株WB800/pBE3.DFE的产孢率仅比对照菌株高9.6%;继续发酵至48 h,菌株WB800/pBE3.DFE的产孢率提高到了83.3%,而对照菌株的产孢率只有57.0%。表明DFE可以提高枯草杆菌的产孢率。在酸性、NaCl、高温、H2O2胁迫下,菌株WB800/pBE3.DFE的耐受性要高于对照菌株。可见,DFE很可能通过调节产孢机制来提高枯草杆菌的耐受性。[结论]该研究初步揭示了DFE在枯草杆菌中的生理功能,为DFE的应用奠定了理论基础。

关键词(KeyWords)： 豆豉纤维酶;枯草杆菌;产孢率;耐受性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30970044)

作者(Author): 陈亮;崔堂兵;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.22.128

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