RP-HPLC-UVD法测定莽吉柿中α-倒捻子素的含量Determination of Alpha-mangostin in Pericarpium Garciniae mangostanae L.by RP-HPLC-UVD
赵岩;刘金平;卢丹;李平亚;张连学;
摘要(Abstract)：
[目的]建立一种测定莽吉柿(Garcinia mangostana L.)中α-倒捻子素(alpha-mangostin)含量的方法。[方法]采用反相高效液相色谱-紫外检测法(RP-HPLC-UVD)测定。色谱条件:迪马公司DiamonsilTMC18色谱柱(250.0mm×4.6mm,5.0μm);流动相,甲醇-水(83∶17);流速,1.0ml/min;柱温,25℃;紫外检测波长,317nm。[结果]在选定的色谱条件下,反相高效液相色谱-紫外检测法符合精密度、准确度和线性(R2>0.999)的基本要求,加样回收率为98.9%,RSD为1.87%。3批莽吉柿样品中α-倒捻子素含量分别为36.4、37.3、38.5mg/g,平均含量为37.4mg/g。[结论]该方法简便,准确,灵敏度高,重现性好,可用于莽吉柿中α-倒捻子素含量的测定。
关键词(KeyWords)： 莽吉柿;α-倒捻子素;双苯吡酮;反相高效液相色谱
基金项目(Foundation):
作者(Author): 赵岩;刘金平;卢丹;李平亚;张连学;
Email:
DOI: 10.13989/j.cnki.0517-6611.2010.11.085
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