王超;李昕荷;张瑞芝;韩悦;齐波;王玉成;

• 1:东北林业大学林木遗传育种国家重点实验室

摘要(Abstract)：

[目的]研究刚毛柽柳水通道蛋白ThPIP基因启动子的克隆及其活性分析。[方法]采用染色体步移技术,克隆到刚毛柽柳ThPIP基因起始密码子上游1 241 bp启动子序列,并通过PLACE和PlantCARE预测启动子序列中所包含的主要顺式作用元件。将该启动子替换pCAMBIA1301上的35S启动子,构建融合表达基因proThPIP∷GUS,通过基因枪瞬时转化烟草,并进行GUS组织化学染色。[结果]该启动子具有活性,能够驱动GUS基因的表达。[结论]为进一步鉴定该启动子中的顺式作用元件及相互作用的调控因子奠定基础,从而为揭示刚毛柽柳ThPIP基因的分子调控机制提供依据。

关键词(KeyWords)： 刚毛柽柳;ThPIP基因;启动子克隆;瞬时表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(31300571);; 东北林业大学大学生创新训练计划项目(201310225087)

作者(Author): 王超;李昕荷;张瑞芝;韩悦;齐波;王玉成;

Email:

DOI: 10.13989/j.cnki.0517-6611.2014.24.004

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