周惠;辛培尧;赵丽华;原晓龙;陈杰;周军;

• 1:西南林业大学林学院
• 2:西南林业大学西南山地森林保育与利用省部共建教育部重点实验室
• 3:中国科学院上海生命科学研究院植物生理生态研究所

摘要(Abstract)：

[目的]分析重金属结合蛋白基因hmbp在转基因拟南芥中的功能。[方法]利用PCR方法从抗辐射奇异球菌(Deinococcus radiodurans)中克隆hmbp基因,并用沾花法转入拟南芥,用氯化镉处理得到的阳性植株后,检测转基因植株中的丙二醛、脯氨酸、可溶性糖、叶绿素含量、相对电导率及SOD、POD活性。[结果]与野生型相比,镉处理后的转基因拟南芥中脯氨酸、可溶性糖含量及SOD、POD活性增加较多,丙二醛含量增加较少,叶绿素含量减少较少。[结论]研究表明hmbp转基因拟南芥对镉具有很高的抗逆性。

关键词(KeyWords)： 植物修复;镉;转基因拟南芥;hmbp

Abstract:

Keywords:

基金项目(Foundation): 上海市科技计划项目(CIE01G1106)资助

作者(Author): 周惠;辛培尧;赵丽华;原晓龙;陈杰;周军;

Email:

DOI: 10.13989/j.cnki.0517-6611.2013.10.091

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