纪冬辉;王义;王昱;王康宇;蒋世翠;张美萍;

• 1:吉林农业大学生命科学学院

摘要(Abstract)：

食药用菌不仅有其独特的风味和口感,还具有很好的营养和药用价值,具有广阔的研究应用前景。文中综述了食药用菌的原生质体融合及遗传转化方法的研究进展,并分析了存在问题,最后对食药用菌遗传转化研究的发展进行了展望。

关键词(KeyWords)： 食药用菌;转化;灵芝;启动子;选择标记

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 纪冬辉;王义;王昱;王康宇;蒋世翠;张美萍;

Email:

DOI: 10.13989/j.cnki.0517-6611.2013.10.118

参考文献(References)：

• [1]WESSELS J G H,DE VRIES O M H.Wall structure,wall degradation,pro-toplast liberation and wall regenerat ion in Schizophyllum commune[C]//Yeast mould and plant protoplasts1.London:Academic Press,1973:295-3061.
• [2]彭卫红,郑林用,甘炳成,等.灵芝原生质体融合HH系列菌株的选育研究[J].西南农业学报,2006,19(3):498-501.
• [3]任轩,贾乐,杨凤苓,等.食用菌原生质体融合育种研究进展[J].生物技术通报,2008(2):42-44,53.
• [4]PEBERDY J F.Fungi without coats-protoplasts as tool for mycological re-search[J].Mycologial Research,1989,13(1):1-10.
• [5]曾荣,刘祖同,张树庭.食用菌灭活原生质体电融合及属间融合产物的鉴定分析[J].微生物学报,1995,35(2):119-151.
• [6]肖在勤,谭伟.金针菇与凤尾菇科间原生质体融合研究[J].食用菌学报,1998,5(1):6-12.
• [7]王澄澈,梁枝荣.凤尾菇和香菇原生质体非对称融合[J].菌物系统,2000,19(3):413-415.
• [8]梁枝荣,安沫平,通占天.凤尾菇和裂褶菌原生质体非对称融合研究[J].菌物系统,2001,20(1):111-115.
• [9]李省印,李孟楼,胡彩霞,等.平菇种内原生质体分离与融合杂交育种技术及应用[J].西北农业学报,2004,13(14):146-151.
• [10]彭卫红,肖在勤,甘炳成.金针菇转核育种研究[J].食用菌学报,2001,8(3):1-5.
• [11]彭卫红,肖在勤,郑林用,等.长根菇与鸡纵菌属间不对称融合后代特性研究[J].食用菌学报,2002,9(1):1-5.
• [12]彭卫红,郑林用,甘炳成,等.灵芝原生质体融合HH系列菌株的选育研究[J].西南农业学报,2006,19(3):498-501.
• [13]贺建超,贺榆霞.木耳灭活原生质体融合育种研究[J].中国食用菌,2002,22(5):16-17.
• [14]王淑珍,白晨,高雁,等.松茸与香菇原生质体融合的研究[J].食用菌,2003,25(2):9-10.
• [15]白淑珍,白晨,范俊,等.灵芝与糙皮侧耳原生质体融合子基因组RAPD分析[J].食用菌学报,2003,10(1):1-5.
• [16]范雷法,潘慧娟食用菌属间原生质体融合研究初报[J].中国食用菌,2005,24(5):21-27.
• [17]许美燕,唐传红,张劲松,等,利用SRAP和ISSR建立快速鉴定灵芝属菌株的SCAR标记[J].菌物学报,2008,27(5):707-717.
• [18]曹文芩,郭顺星,徐锦堂,等.灵芝原生质体制备再生及融合的研究[J].菌物系统,1998,17(1):52-56.
• [19]刘方.灵芝原生质体单核化杂交育种[D].福州:福建农业大学,2009.
• [20]苗敬芝,朱芹波,吕兆启,等.利用生化方法鉴定灵芝原生质体融合子的研究[J].食品科技,2010,35(4):259-261.
• [21]全卫丰,何静霞,汪洁,等.原生质体融合选育富硒高产灵芝的研究[J].食用菌,2012(3):19-21.
• [22]武小霞,李文滨,张淑珍.我国大豆转基因研究进展[J].大豆科学,2005,24(2):144-149.
• [23]MUNOZ RIVAS A,SPECIIT C A,DRUMMOND B J,et al.Transformationof the basidiomycele Schizophllum commune[J].Mol Gen Genel,1986,205(1):103-106.
• [24]胡乐琴,潘迎捷.食用菌分子转化研究状况[J].中国食用菌,2006,25(4):7-10.
• [25]李刚,王强,刘秋云,等.利用PEG法建立药用真菌灵芝的转化系统[J].菌物学报,2004,23(2):255-261.
• [26]杨炜,黄大年,王金霞.以潮霉素抗性为选择标记的稻瘟病原生质体转化[J].遗传学报,,1994,21(4):305-312.
• [27]郭慧,COOLEY R N.粟长蠕孢菌的原生质体转化[J].真菌学报,1990,9(4):286-292.
• [28]PENG M,SINGH N K,LEMKE P A.Recovery of recombinant plasmidsfrom Pleurotus ostreatus transformants[J].Curr Genet,1992,22:53-59.
• [29]LI G,LI R,LIU Q,et al.A highly efficient polyethylene glycol-mediatedtransformation method for mushrooms[J].FEMS Microbiol Lett,2006(256):203-208.
• [30]XU F,ZHAO M W,LI Y X.Cloning and sequence analysis of a glyceral-dehyde-3-phosphate dehydrogenase gene from Ganoderma lucidum[J].J Microbiol,2006,44(5):515-522.
• [31]徐飞.灵芝三磷酸甘油醛脱氢酶基因的克隆及转化体系的构建[D].南京:南京农业大学,2007.
• [32]郭丽琼,刘二鲜,王杰,等.高效银耳芽孢遗传转化体系的建立[J].中国农业科学,2008,41(11):3728-3734.
• [33]ZHAO F Y,LIN J F,ZENG L X,et al.Improvement in fruiting body yieldby introduction of the Ampullaria crossean multi-functional cellulasegene into Volvariella volvacea[J].Biores our Technol,2010,101(16):6482-6486.
• [34]孙丽,林忠平.PEG介导香菇的转化[J].植物学报:英文版,2001,43(10):43-46.
• [35]LI G,LI R,LIU Q.A highly efficient polyethylene glycol-mediated trans-formtion method for mushrooms[J].FEMS Microbiol Lett,2006,256(2):203-208.
• [36]THIERRY NOEL,JECQUES LABARERE,HOMOLOGOUS.Transforma-tion of the edible basidiomycete Agrocybe aegerita with theURA1 gene:characterization of integrative events and of rearranged free plasmids intransformants[J].Curr Genet,1994,25:432-437.
• [37]SAMBROOK J,FRISTSCH E F,MANIATIS T.Molecular cloing-A labo-ratory manual[M].New York:Cold Spring Harbor Laboratory Press,1989:50.
• [38]刘昌燕,赵培静,汪军,等.生防真菌遗传转化方法研究进展[J].中国植保导刊,2009(1):13-16.
• [39]燕克勤,朱宝成,赵会良,等.电击法介导的紫孢侧耳原生质体转化[J].生物工程学报,1996,12(1):40-44.
• [40]贾建航,刘振岳,泰丽芳,等.香菇DNA导入平菇原生质体及转化子鉴定研究[J].食用菌学报,1997,4(4):5-10.
• [41]潘迎捷.国际食用菌研究进展[J].吉林农业大学学报,1998,20(S1):64-66.
• [42]SUN L,CAI H Q,XU W H,et al.Efficient transformation of the medicinalmushroom Ganoderma lucidum[J].Plant Molecular Biology Reporter,2001,19:383.
• [43]张新涛,韩春,吴立宇,等.灵芝原生质体的分离及其转化[J].遗传,2001,23(5):458-462.
• [44]ZHANG J,NIE S W,SHAN L,et al.Transformation of metallothioneingene into mushroom protoplasts by application of electroporation[J].ActaBotanica Sinica,2002,44(12):1445-1449.
• [45]郭丽琼,柳永,赵姝娴,等.银耳芽孢完整细胞高效转化体系的建立[J].中国科学C:辑生命科学,2008,38(10):974-981.
• [46]KUO C Y,CHOU S Y,HUANG C T.Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transfor-mation in Flammulina velutipes[J].Appl Microbiol Biotechnol,2004,65(5):593-599.
• [47]KUO C Y,HUANG C T.A reliable transformation method and heterolo-gous expression ofβ-glucuronidase in Lentinula edodes[J].J MicrobiolMeth,2008,72(2):111-115.
• [48]KLEIN T M,WOLF E D,WU R,et al.High velocity microprojectiles fordelivering nucleic acids into living cells[J].Nature,1987,327(6117):70-73.
• [49]SUNAGAWA M,MAGAE Y,YUMI M.Transformation of the edible mush-room Pleurotus ostreatus by particle bombardment[J].FEMS MicrobiolLett,2002,211(2):143-146.
• [50]SUNAGAWA M,MURATA H,MIYAZAKI Y,et al.Transformation of themycorrhizal basidiomycetes,Suillus grevillei and S.bovinus,by particlebombardment[J].Biosci Btiotechnol Biochem,2007,71(1):47-50.
• [51]SUNAGAWA M,MURATA H,MIYAZAKI Y,et al.Transformation of Lyo-phllum decastes by particle bombardment[J].Mycoscience,2007,48(3):195-197.
• [52]郭丽琼,杨飞芸,熊盛,等.草菇基因枪法遗传转化体系的建立[J].园艺学报,2005,32(5):828-833.
• [53]郭丽琼,林俊芳,熊盛,等.抗冷冻蛋白基因遗传转化草菇的研究[J].微生物学报,2005,45(1):39-43.
• [54]VAN DE RHEE M D,MENDES O,WERTEN M W,et al.Highly effici-enthomologous integration via tandem exo-β-1,3-glucanase genes in thecommon mushroom[J].Agaricus Bisporus Curr Genet,1996,30:166-173.
• [55]SATO T,HIRANO T,YAEGASHI K,et al.Transformation of the ediblebasidiomycete lentinus edodes by restriction enzymemediated DNA inte-gration of plasmid DNA Biosci[J].Biotechnol Biochem,1998,62(12):2346-2358.
• [56]KIM SUN KYUNG,SONG JAEMAHN,CHOI HYOUNG T.Genetic trans-formation and mutant isolation in Ganoderma lucidum by restriction en-zyme-mediated integration[J].FEMS,2004,233:201-204.
• [57]谢宝贵,卢启泉,饶永斌,等.人胰岛素基因的人工合成及转化银耳的研究[J].食用菌学报,2007,14(2):1-8.
• [58]SCHUNCN F H J.Highly-effient transformation of the homobasidiomyceteS.commune to Phleomycin resistance[J].Curr Gent,1994,26:179-183.
• [59]傅容昭,孙勇如,贾士荣.植物遗传转化技术手册[M].北京:中国科学技术出版社,1994.
• [60]VAIN P.Thirty years of plant transformation technology development[J].Plant Biotechnol J,2007,5(2):221-229.
• [61]MICHIELSE C B,HOOYKAAS P J,VAN DEN HONDEL C A,et al.Agrobacterium-mediated transformation as a tool for functional genomicsin fungi[J].Curr Genet,2005,48(1):1-17.
• [62]SUN W,LIU L,HU X,TANG J,et al.Generation and identification ofDNA sequence flanking T-DNA integration site of Trichoderma atroviridemutants with high dichlorvos-degrading capacity[J].Bioresour Technol,2009,100(23):5941-5946.
• [63]CHEN E C,SU Y H,KANAGARAJAN S,et al.Development of an activa-tion tagging system for the basidiomycetous medicinal fungus Antrodiacinnamomea[J].Mycol Res,2009,113(3):290-297.
• [64]CHEN X,STONE M,SCHLAGNHAUFER C,et al.A fruiting body tissuemethod for efficient Agrobacterium-mediated transformation of Agaricusbisporus[J].Appl Environ Microbiol,2000,66(10):4510-4513.
• [65]THOMAS S P M,LAVRIJSSEN B.Transformation of the cultivated mush-room Agaricus bisporus using T-DNAfrom Agrobacterium tumefaciens[J].Curr Genet,2001,39:35-39.
• [66]WANG J,GUO L,ZHANG K,et al.Highly efficient Agrobacterium-media-ted transformation of Volvariella volvacea[J].Bioresour Technol,2008,99(17):8524-8527.
• [67]陈美元,廖剑华,郭仲杰,等.双孢蘑菇耐热相关基因的表达载体构建及转化研究[J].菌物学报,2009,28(6):797-801.
• [68]喻义赣.香菇农杆菌接到转化条件优化及BCA基因转化研究[D].福州:福建农林大学,2009.
• [69]郭益童.农杆菌介导人胰岛素基因BCA转化银耳研究[D].福州:福建农林大学,2010.
• [70]SHI L,FANG X,LI M J,et al.Development of a simple and efficienttransformation system for the basidiomycetous medicinal fungus Ganoder-ma lucidum[J].World J Microbiol Biotechnol,2012,28:283-291.
• [71]邱龙新.食用蕈菌的基因工程研究现状[J].龙岩师专学报,2004,22(6):61-64.
• [72]柳永.灵芝药效基因及启动子的克隆的研究[D].儋州:华南热带农业大学,2004.
• [73]ROYER J C,HORGEN P A.Towards a transformation ofthe edible basid-iomycete Agrocybe aegerita with theURA1 gene characterization of inte-grative events andrearranged free plasmids in transformation[J].CurrentGenetics,1991,22(1):53-59.
• [74]廖剑华,王泽生,陈美元,等.双孢蘑菇电击转化育种技术研究[J].菌物学报,2005(24):121-124.