星豹蛛乙酰胆碱酯酶(AChE)活性测定及药剂对其活性的抑制作用Determination of the Activity of Acetylcholinesterase in Pardosa astrigera and the Inhibition Effect of Pesticide on Its Activity
马敏;张宾;葛衍珍;李生才;
摘要(Abstract)：
[目的]系统研究星豹蛛(Pardosa astrigera)乙酰胆碱酯酶(AChE)的性质,确定其抗药性的发生和发展及其抗性水平,建立快速、准确的AChE活性检测方法。[方法]采用正交试验设计确定星豹蛛不同部位AChE活性测定的最优条件组合,并在此基础上进一步研究豹蛛AChE的体躯分布及其对4种常用药剂的敏感度。[结果]星豹蛛头胸部、腹部和附肢AChE活性测定的最优条件组合是:酶浓度分别为12、18、29 g/L;底物浓度分别为0.6、1.0、1.0 mmol/L;反应体系pH值均为7.0;反应温度分别为30、35、35℃;反应时间均为5min。AChE主要分布在星豹蛛的头胸部,提取液中含有Triton X-100时,AChE的比活力要比无Triton X-100时大。灭多威、辛硫磷、高效氯氰菊酯、毒死蜱对星豹蛛头胸部中AChE的抑制中浓度(IC50)分别为7.76×10-5、1.76×10-4、4.12×10-4、4.94×10-4mol/L。[结论]星豹蛛体内AChE是膜结合的;4种药剂对星豹蛛头胸部中AChE的抑制具有很好的剂量效应,表明星豹蛛头胸部AChE可以作为环境中有机磷、氨基甲酸酯和拟除虫菊酯类农药污染的生物化学标记。
关键词(KeyWords)： 星豹蛛;乙酰胆碱酯酶;生物化学标记;药剂敏感度;抑制中浓度
基金项目(Foundation): 山西省留学基金(2009042);; 山西省青年科技研究基金(2008021041)
作者(Author): 马敏;张宾;葛衍珍;李生才;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.06.145
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