蒋小平;蔡俊鹏;

• 1:华南理工大学生物科学与工程学院
• 2:华南理工大学轻工与食品学院

摘要(Abstract)：

[目的]研究噬菌蛭弧菌BDYJAL对新鲜草鱼片大肠杆菌生长的控制作用。[方法]进行了蛭弧菌BDYJAL对32株潜在致病菌的裂解试验;并通过设置3个组别:蛭弧菌低浓度组(蛭弧菌∶宿主=1∶1)、蛭弧菌高浓度组(蛭弧菌∶宿主=10∶1)和非添加蛭弧菌对照组,进行了蛭弧菌BDYJAL对草鱼片大肠杆菌的生长控制试验。[结果]蛭弧菌BDYJAL对32株潜在致病菌的裂解率达到了93.8%;鱼片大肠杆菌控制试验中,与对照组相比2个试验组对大肠杆菌的消除控制存在显著差异(P<0.05),但这2个试验组在0~36 h内的消除效果无显著差异(P>0.05)。[结论]蛭弧菌BDYJAL能有效控制新鲜草鱼片的大肠杆菌生长。

关键词(KeyWords)： 蛭弧菌;大肠杆菌;新鲜草鱼片;生长控制

Abstract:

Keywords:

基金项目(Foundation): 广东省海洋渔业科技推广专项(A200899H02);; 广东省科技计划-省部产学研项目(2009B090300270);; 国家自然科学基金项目(40776091)

作者(Author): 蒋小平;蔡俊鹏;

Email:

DOI:

参考文献(References)：

• [1]BASTI AA,MISAGHI A,SALEHI TZ,etal.Bacterial pathogens in fresh,smoked and salted Iranian fish[J].Food Control,2006(17):183-188.
• [2]张梁.噬菌蛭弧菌对草鱼池水质及细菌群落的影响研究[J].水生态学杂志,2009,2(1):6-10.
• [3]严建民.辐照食品的卫生安全性研究现状[J].核农学报,2010,24(1):88-92.
• [4]WOLKOFFP,SCHNEIDERT,KILDESJ,etal.Riskin cleaning:chemi-cal and physical exposure[J].The Science ofthe Total Environment,1998,215(1/2):135-156.
• [5]杨吉霞,徐丽,蔡俊鹏.海水养殖中应用蛭弧菌控制病原菌的前景与问题[J].湛江海洋大学学报,2004,24(3):79-84.
• [6]LYNCHMF,TAUXE R V,HEDBERGC W.The growingburden of food-borne outbreaks due to contaminated fresh produce risks and opportunities[J].Epidemiol Infect,2009,137:307-315.
• [7]宋志萍,蔡俊鹏,王志,等.蛭弧菌的分离及其生长条件和裂解能力的研究[J].微生物学报,2005,45(4):571-575.
• [8]LUF,CAI J.The protective effect ofBdellovibrio-and-like organisms(BA-LO)on tilapia fish fillets againstSalmonella entericassp.serovar Typhi-murium[J].Applied Microbiology,2010,51(6):625-631.
• [9]HE J W.Chemical and biological transformations for detoxification of tri-chothecene mycotoxins in human and animal food chains:a review[J].Trends in Food Science&Technology,2010,21:67-76.
• [10]HEINITZML,RUBLERD,WAGNERDE,etal.Incidence ofSalmonel-lain fish and seafood[J].Food Prot,2000,63:579-592.
• [11]JALALI M,ABEDI D,POURBAKHSHS A,GHOUKASIN K.PrevalenceofSalmonellaspp.in rawand cooked foods in Isfashahan-Iran[J].FoodSaf,2008,28:442-452.
• [12]ALLENDE A,MCEVOYJ,TAOY,et al.Antimicrobial effect of acidifiedsodiumchlorite,sodium chlorite,sodium hypochlorite,and citric acid onEscherichia coliO157:H7 and natural micro-flora of fresh-cut cilantro[J].Food Control,2009,20:230-234.
• [13]孟宪梅.食品的大肠杆菌O157污染检测及预防控制[J].肉品卫生,2004,245(11):30-33.
• [14]肖荣海.进口冰冻水产品大肠杆菌O157:H7的检测与鉴定[J].云南畜牧兽医,2004(2):5-6.
• [15]KOTTAPALLI B,WOLF-HALL,SCHWARZ C E P.Effect of electronbeamradiation on the safety and quality ofFusariuminfected maltingbarley[J].International Journal of Food Microbiology,2006,110:224-231.
• [16]彭宗辉,蔡俊鹏,吴冰,等.两株海洋蛭弧菌的分离及生物学性质[J].微生物学报,2008,48(11):1425-1431.
• [17]蔡俊鹏,芦志龙,袁尔东.蛭弧菌对溶藻弧菌的消除作用研究[J].现代食品科技,2009,25(2):126-129.
• [18]韩韫,蔡俊鹏,宋志萍,等.应用蛭弧菌清除海产品潜在致病弧菌的研究[J].水产科学,2005,24(11):23-25.
• [19]STARR MP,STOLP H.Bdellovibrio methodology[J].In Methods in Mi-crobiology,1996,18:217-244.
• [20]FRATAMICOP M,COOKE P H.Isolation ofBdellovibriosthat prey onEscherichia coil015:H7 andSalmonellaspecies and application for re-moval of prey from stainless steel surface[J].Food Saf,1996,16:161-173.