李静雯;欧巧明;张正英;

• 1:甘肃省农业科学院生物技术研究所

摘要(Abstract)：

针对RAPD分析技术在小麦分子育种研究中所出现的具体问题,对其在小麦遗传变异分析、目的基因的标记与定位、外源染色体的检测、品种鉴定、遗传图谱的构建、近缘种属间的遗传关系与遗传多样性研究等方面的最新进展和应用进行了综述,为小麦分子标记辅助选择育种等相关研究提供有益的借鉴。

关键词(KeyWords)： RAPD;小麦;分子育种;分子标记

Abstract:

Keywords:

基金项目(Foundation): 甘肃省科技攻关项目“小麦生物技术育种及种质创新研究”(GS035-A41-001-03)

作者(Author): 李静雯;欧巧明;张正英;

Email:

DOI:

参考文献(References)：

• [1]DEVOS K M,GALE M D.The use of random amplified polymorphismDNAmarkers in wheat[J].Theoretical and Applied Genetics,1992,84:567-572.
• [2]裴新梧,崔凯荣,孔英珍.导入高梁DNA选育丰产、抗逆小麦新品系及其RAPD分子验证[J].兰州大学学报,1996,35(2):131-135.
• [3]王黎明.外源DNA导入高粱及其后代的RAPD分子验证[J].中国农学通报,2002,18(3):19-21.
• [4]郑红军,于元杰,尹承佾.外源DNA导入小麦的RAPD验证及遗传分析[J].西北植物学报,2002,22(4):758-765.
• [5]雷勃钧,李希臣,卢翠华,等.外源野生大豆DNA导入栽培大豆及RAPD分子验证[J].中国科学(B辑),1994,24(6):596-601.
• [6]伍玲,李平,刘熔山.外源导入水稻的变异材料随机扩增多态性分析[J].西南农业学报,1998,11(4):28-32.
• [7]鲍晓明,黄百渠,李松涛,等.用RAPD技术鉴定两个小冰麦易位系[J].遗传学报,1993,20(1):81-87.
• [8]毛龙,胡含,朱立煌,等.应用RAPD分析一个抗条锈病的小麦-黑麦易位系[J].科学通报,1994,39(22):2087-2090.
• [9]IQBALMJ.Identificationofthe1RSryechromosomal segmentinwheatbyRAPD analysis[J].Theoretical and Applied Genetics,1995,91:1048-1053.
• [10]FRANCISHA.Conversion of a RAPDgenerated PCRproduct,containinga novel dispersed repetitive element into a fast and robust assay for thepresence of ryechromatin in wheat[J].Theoretical and Applied Genetics,1995,90:636-642.
• [11]陈建莉,薛秀庄.用Landon二体代换系建立小麦染色体RAPD标记[J].遗传学报,1996,23(1):32-39.
• [12]孙其信.节节麦D染色体组特异RAPD标记[J].中国农业大学学报,1996,1(1):34-36.
• [13]KINGI P.Characterization ofThinopyrumbessarabicumchromosome seg-ments in wheat using randomamp lified polymorphic DNAs(RAPDs)andgenomic insituhybridization[J].Theoretical and Applied Genetics,1993,86:895-900.
• [14]李松涛,张忠延,王斌,等.用新的分子标记方法(RAPD)分析小麦抗白粉病基因Pm4a的近等基因系[J].遗传学报,1995,22(2):103-108.
• [15]PROCUNIERJ D.PCRbased RAPD-DGGEmarkers linked toleafrustresistance genesLr29 and 25 in wheat[J].Journal of Genetics&Breeding,1995,49:87-91.
• [16]SCHACHERMAYR G,MESSMER MM,FEUILLETC,et al.Identifi-cation of molecular markers linked to theAgropyron elongatumderivedleaf rust resistance geneLr24 in wheat[J].Theoretical and AppliedGenetics,1995,90:982-990.
• [17]SCHACHERMAYR G,SIEDLER H,GALE M D,et al.Identificationand localization of molecular markers linked to theLr9 leaf resistancegene of wheat[J].Theoretical and Applied Genetics,1994,88:110-115.
• [18]DWEIKATI.Pedigree assessment using RAPD-DGGE in cereal cropspecies[J].Theoretical and Applied Genetics,1993,85:497-505.
• [19]DEMEKE T,LAROCHE A,GAUDET D A,et al.A DNA marker fortheBt210 common bunt resistance gene in wheat[J].Genome,1996,39:51-55.
• [20]QI LL.Identification,maping,and application of polymorphic DNAas-sociated with resistance genePm21 of wheat[J].Genome,1996,39:191-197.
• [21]HARTL L,WEISS H,STEPHAN U,et al.Molecular identification ofpowdery mildewresistance gene in common wheat[J].Theoretical andApplied Genetics,1995,90:601-606.
• [22]宋书娟,李雅轩,郭平仲,等.小麦异交群体长期选择早代的RAPD多态性分析[J].首都师范大学学报,1999,20(3):53-61.
• [23]朱其洪,贾旭,王京兆,等.用RAPD方法获得与小麦BYDV抗性基因连锁的分子标记[J].科学通报,1995,40(19):1709-1801.
• [24]王京兆,王斌,徐琼芳,等.用RAPD方法分析水稻光敏不育基因[J].遗传学报,1995,22(1):53-58.
• [25]JOSHI C P.Application of the RAPD forthe detection of polymorphismamong wild and cultivatedtet raploid wheats[J].Genome,1993,36(3):602-609.
• [26]JOSHI C P.RAPD analysis based intervarietal genetic relationships a-mong hexaploid wheats[J].Plant Science(Limerick),1993,93(1/2):95-103.
• [27]WEI J Z,WANGRC.Genome and species specificmarkers and genomerelationships of diplod perennial species in Triticease based on RAPDanalyses[J].Genome,1995,38:1230-1236.
• [28]DHALIWALHS.Genetic diversity in diplod and hexaploid wheats asrevealed by RAPD markers[J].Crop Improvment,1993,20(1):17-20.
• [29]HE S,MACKENIC S.Detection of DNA sequence polymorphisms a-mong wheat varieties[J].Theoretical and Applied Genetics,1992 84:573-578.
• [30]李丽,郑晓鹰,KLCKE E.利用RAPD分子标记对番茄杂交种纯度的鉴定研究[J].广西植物,2003,23(2):149-154.