沈佳;章振华;姜北宇;李林;景小冬;张建伟;

• 1:北京市农林科学院畜牧兽医研究所

摘要(Abstract)：

[目的]探讨2株H9N2分离毒株与其他参考毒株的全基因组遗传变异关系。[方法]采用RT-PCR技术扩增了2株H9N2亚型禽流感病毒分离株8个基因片段的全基因序列,并对所得序列与6个参考毒株进行了同源性比较及遗传进化树分析。[结果]2个毒株与6个参考毒株全基因组同源性在91.1%～95.4%,PG08与C/BJ/1/94的全基因序列的核苷酸同源性最高为91.3%;而ZD06与D/HK/Y280/97最高为92.3%。2个分离株的HA基因裂解位点均为PARSSR/GLF,均为H9N2低致病力禽流感病毒。[结论]ZD06株与C/Pak/2/99的亲缘关系最近,属欧亚种系;PG08株与其他毒株亲缘关系稍远,它可能是不同亚支基因重排的产物。

关键词(KeyWords)： 禽流感病毒;H9N2;全基因组;序列分析

Abstract:

Keywords:

基金项目(Foundation): 国家973资助项目子课题(2005CB523001)

作者(Author): 沈佳;章振华;姜北宇;李林;景小冬;张建伟;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.09.003

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