中国鹿亚科动物mtDNA控制区序列差异及其遗传分化Sequence Difference of Mitochondrial DNA Control Region and Genetic Differentiation of Cervinae in China
涂剑锋;邢秀梅;徐佳萍;杨颖;杨福合;
摘要(Abstract):
[目的]探讨中国鹿亚科动物的遗传分化。[方法]通过PCR直接测序的方法获得我国鹿亚科物种线粒体DNA控制区(D-loop)全序列,结合GenBank检索到的鹿亚科其他动物同源序列,用生物软件进行统计分析。[结果]我国鹿亚科动物D-loop区序列全长在921~1 072 bp,碱基T、A、C、G的平均含量分别为32.1%、30.2%、22.7%及15.0%,各物种间的遗传距离范围在0.062~0.106之间,处于属间差异。系统进化树结果表明梅花鹿、马鹿和白唇鹿亲缘关系较近,它们与水鹿构成一组进化枝,坡鹿与麋鹿构成一组进化枝,豚鹿单独构成一个进化枝。[结论]该研究支持麋鹿和豚鹿并入鹿属的观点,我国的鹿亚科动物的分化时间约为155~260万年。
关键词(KeyWords): 鹿亚科;控制区;序列差异;遗传分化
基金项目(Foundation): 国家科技支撑计划(2007BAI38B03);; 国家自然科技资源平台项目(No.2004DKA30460)
作者(Author): 涂剑锋;邢秀梅;徐佳萍;杨颖;杨福合;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.02.006
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