从生物转化液中分离提取11-α-羟基-坎利酮的工艺研究Study on Extraction Methods of 11-α-Hydroxy-Canrenone from Biotransformation Broth
崔凤杰;张天真;黄达明;孙文敬;钱静亚;
摘要(Abstract)：
[目的]考察了4种分离提取方法对生物转化液中转化产物11-α-羟基-坎利酮提取率的影响。[方法]4种分离提取方法分别为文献法、浸泡法、洗脱法和匀浆法。[结果]11-α-羟基-坎利酮主要游离于转化液或附着于菌丝体外表面,洗脱法和浸泡法对11-α-羟基-坎利酮具有较好的分离效果。采用400 ml乙酸乙酯洗脱,11-α-羟基-坎利酮提取率为96.0%;经乙酸乙酯浸泡90 min,11-α-羟基-坎利酮提取率达到98.8%。[结论]该方法简单高效,具有工业化应用潜力。
关键词(KeyWords)： 11-α-羟基-坎利酮;提取;生物转化
基金项目(Foundation): 国家自然基金项目(31101269);; 镇江市工业科技支撑项目(GY2011006);; International Foundation for Science(F 4930-1);; 江西省生物发酵食品添加剂工程技术研究中心;; 江西省科技条件平台建设项目(2010DTZ01900);; 江苏大学学生科研立项资助项目(10A229,10A080)
作者(Author): 崔凤杰;张天真;黄达明;孙文敬;钱静亚;
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DOI:
参考文献(References)：
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