吉尔吉斯白桦BkWRKY1基因克隆与序列分析The Analysis and Cloning of BkWRKY1 Gene of Betula kirghisorum
田玉富;崔璨;谢龙飞;李桂英;杨成君;
摘要(Abstract):
从吉尔吉斯白桦转录组文库测序中获得白桦BkWRKY1转录因子的cDNA序列,该序列包含2 189个碱基。序列比对和同源性分析表明,该基因cDNA序列包含1 728 bp的开放阅读框,可编码575个氨基酸,5'非翻译区(UTR)为119 bp,3'非翻译区(UTR)为342bp。该基因属于WRKY transcription factor家族,含有2个WRKY结构域,蛋白分子量为62.483 kD,理论等电点为7.32,负电荷残基(Asp+Glu)总数为62个,正电荷残基(Arg+Lys)总数为62个。蛋白不含有信号肽,具有一定的亲水性,为亲水蛋白,无跨膜结构。同源性比较与进化树分析表明,吉尔吉斯白桦WRKY蛋白与大豆和蒺藜苜蓿的WRKY蛋白在进化上关系较近。该基因在0.6%NaHCO3胁迫处理后表达量增加,为上调表达基因。
关键词(KeyWords): 吉尔吉斯白桦;WRKY转录因子;生物信息学分析
基金项目(Foundation): 中央高校基本科研业务费专项资金项目(DL12CA13);; 东北林业大学大学生创新训练项目(201310225101)
作者(Author): 田玉富;崔璨;谢龙飞;李桂英;杨成君;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.23.006
参考文献(References):
- [1]ISHIGURO S,NAKAMURA K.Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5'upstream regions of genes coding for sporamin andβ-amylase from sweet potato[J].Mol Gen Genet,1994,244:563-571.
- [2]RUSHTON P J,MACDONALD H,HUTTLY A K,et al.Members of a new family of DNA-binding proteins bind to a conserved cis-element in the promoters of a-Amy2 genes[J].Plant Molecular Biology,1995,29(4):691-702.
- [3]RUSHTON P J,TORRES J T,PARNISKE M,et al.Interaction of elicitorinduced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes[J].The EMBO Journal,1996,15(20):5690-5700.
- [4]PATER S D,GRECO V,PHAM K,et al.Characterization of a zinc-Dependent transcriptional activator from Arabidopsis[J].Nucleic Acids Resarch,1996,24(23):4624-4631.
- [5]EULGEM T,RUSHTON P J,SCHMELZER E,et al.Early nuclear events in plant defense signaling:rapid gene activation by WRKY transcription factors[J].EMBO Journal,1999,18(17):4689-4699.
- [6]贾翠玲,侯和胜.植物WRKY转录因子的结构特点及其在植物防卫反应中的作用[J].天津农业科学,2010,16(2):21-26.
- [7]EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):200-206.
- [8]MORTAZAVI A,WILLIAMS B A,MCCUE K,et al.Mapping and quantifying mammalian transcriptomes by RNA-Seq.Nat Methods,2008,5(7):621-628.
- [9]王育娜.辣椒WRKY转录因子cDNA的分离与功能鉴定[D].福州:福建农林大学,2008.
- [10]薛华,张红岩,李小艳,等.油菜矮秆突变WRKY转录因子cDNA克隆及表达分析[J].西北植物学报,2008,28(3):452-458.
- [11]黎华.玉米WRKY家族转录因子基因ZmWRKY33的克隆及功能分析[D].扬州:扬州大学,2011.
- [12]潘林杰.番木瓜WRKY转录因子的基因表达谱及其功能的初步研究[D].武汉:华中农业大学,2011.
- [13]江腾,林勇祥,刘雪,等.苜蓿全基因组WRKY转录因子基因的分析[J].草业学报,2011,20(3):211-218.
- [14]何红升.杨树全基因组WRKY基因的鉴定及表达分析[D].合肥:安徽农业大学,2012.
- [15]许瑞瑞,张世忠,曹慧,等.苹果WRKY转录因子家族基因生物信息学分析[J].园艺学报,2012,39(10):2049-2060.
- [16]苗立祥,张豫超,杨肖芳,等.森林草莓全基因组WRKY转录因子基因的鉴定与分析[J].核农学报,2012,26(8):1124-1131.
- [17]鄂志国,王磊.水稻WRKY基因家族功能研究进展[J].核农学报,2012,26(5):750-755.
- [18]ZHOU Q Y,TIAN A G,ZOU H F,et al.Soybean WRKY-type transcription factor genes,GmWRKY13,GmWRKY21,and GmWRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants[J].Plant Biotechnology Journal,2008,6(5):486-503.
- [19]DEVAIAH B N,KARTHIKEYAN A S,RAGHOTHAMA K G.WRKY75transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis[J].Plant Physiology,2007,143(4):1789-1801.
- [20]ZHANG J,PENG Y L,GUO Z J.Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants[J].Cell Research,2008,18(4):508-521.
- [21]ROBATZEK S,SOMSSICH I E.A new member of the Arabidopsis WRKY transcription factor family,AtWRKY6,is associated with both senescence-and defence-related processes[J].Plant Journal,2001,28(2):123-133.
- [22]ULKER B,MUKHTAR M S,SOMSSICH I E.The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling Pathways[J].Planta,2007,226(1):125-137.
- [23]JING S J,ZHOU X,SONG Y,et al.Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis[J].Plant Growth Regulation,2009,58(2):181-190.
- [24]SUN C,PALMQVIST S,OLSSON H,et al.A novel WRKY transcription factor,SUSIBA2,participates in sugar Signaling in barley by binding to the sugar-responsive elements of the iso1 promoter[J].Plant Cell,2003,15(9):2076-2092.
- [25]XU Y H,WANG J W,WANG S,et al.Characterization of GaWRKY1,a cotton transcription factor that regulates the sesquiterpene Synthase gene(+)-δ-cadinene synthase-A[J].Plant Physiology,2004,135(1):507-515.
- [26]ZHANG Z L,XIE Z,ZOU X L,et al.A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling Pathway in aleurone cells[J].Plant Physiology,2004,134(4):1500-1513.
- [27]XIE Z,ZHANG Z L,ZOU X L,et al.Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin Signaling in aleurone cells[J].The Plant Journal,2006,46(2):231-242.
- [28]XIE Z,ZHANG Z L,HANZLIK S,et al.Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a Pathway involving an abscisic-acid-inducible WRKY gene[J].Plant Molecular Biology,2007,64(3):293-303.
- [29]ZOU X,NEUMAN D,SHEN Q J.Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells[J].Plant Physiology,2008,148(1):176-186.
- [30]WEI W,ZHANG Y,HAN L,et al.A novel WRKY transcriptional factor from Thlaspi caerulescens negatively regulates the osmotic Stress tolerance of transgenic tobacco[J].Plant Cell reports,2008,27(4):795-803.
- [31]CHEN Y F,LI L Q,XU Q,et al.The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to Low Pi stress in Arabidopsis[J].The Plant Cell Oline,2009,21(11):3554-3566.
- [32]JIANG Y,DEYHOLOS M K.Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses[J].Plant Molecular Biology,2009,69(1/2):91-105.
- [33]WU X,SHIROTO Y,KISHITANI S,et al.Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter[J].Plant Cell reports,2009,28(1):21-30.
- [34]YANG B,JIANG Y Q,RAHMAN M H,et al.Identification and expression analysis of WRKY transcription factor genes in canola(Brassica napus L.)in response to fungal pathogens and hormone treatments[J].BMC Plant Biology,2009,9(1):68.
- [35]KIM K C,LAI Z,FAN B,et al.Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense[J].The Plant Cell Oline,2008,20(9):2357-2371.
- [36]RYU H S,HAN M,LEE S K,et al.A comprehensive expression analysis of the W RKY gene superfamily in rice plants during defense response[J].Plant Cell Reports,2006,25(8):836-847.
- [37]ZHENG Z,QAMAR S A,CHEN Z X,et al.Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens[J].The Plant Journal,2006,48(4):592-605.
- [38]KALDE M,BARTH M,SOMSSICH I E,et al.Members of the Arabidopsis WRKY groupⅢtranscription factors are part of different plant defense signaling pathways[J].Molecular Plant-microbe Interactions,2003,16(4):295-305.
- [39]KNOTH C,RINGLER J,DANGL J L,et al.Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica[J].Molecular Plant-microbe Interactions,2007,20(2):120-128.
- [40]LIU X Q,BAI X Q,QIAN Q,et al.OsWRKY03,a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J].Cell Research,2005,15(8):593-603.
- [41]CATALINO N J,SOMSSICH I E,ROBY D,et al.The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana[J].The Plant Cell Oline,2006,18(11):3289-3302.
- [42]OH S K,YI S Y,YU S H,et al.CaWRKY2,a chili pepper transcription factor,is rap idly induced by incompatible plant pathogens[J].Molecular Cells,2006,22(1):58-64.
- [43]XU X P,CHEN C H,FAN B F,et al.Physical and functional interactions between pathogen-induced Arabidopsis WRKY18,WRKY40,and WRKY60 transcription factors[J].The Plant Cell Oline,2006,18(5):1310-1326.
- [44]LIPPOK B,BIRKENBIHL R P,RIVORY G,et al.Expression of AtWRKY33 encoding a pathogen-or PAMP-responsive WRKY transcription factor is regulated by a composite DNA motif containing W box elements[J].Molecular Plant-microbe Interactions,2007,20(4):420-429.
- [45]ZHENG Z Y,MOSHER S L,FAN B F,et al.Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae[J].BMC Plant Biology,2007,7(2):1-13.
- [46]YANG P Z,CHEN C H,WANG Z,et al.A pathogen-and salicylic acid-induced WRKY DNA-binding activity recognizes the elicitor response element of the tobacco class I chitinase gene promoter[J].The Plant Journal,1999,18(2):141-149.
- [47]YODA H,OGAWA M,YAMAGUCHI Y,et al.Identification of early-responsive genes associated with the hypersensitive response to tobacco mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants[J].Molecular Genetics Genomics,2002,267(2):154-161.
- [48]PARK C J,SHIN Y C,LEE B J,et al.A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to tobacco mosaic virus and Xanthomonas campestris[J].Planta,2006,223(2):168-179.
- [49]CORMACK R S,EULGEM T,RUSHTON P J,et al.Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitorinduced WRKY transcription factors in parsley[J].Biochimica et Biophysica Acta,2002,1576(1):92-100.
- [50]CHEN C H,CHEN Z X.Potentiation of developmentally regulated plant defense r esponse by AtWRKY18,a pathogen-induced Arabidopsis transcription factor[J].Plant Physiology,2002,129(2):706-716.
- [51]LIU X Q,BAI X Q,WANG X J,et al.OsWRKY71,a rice transcription factor,is involved in rice defense response[J].Plant Physiology,2007,164(8):969-979.
- [52]ISHIDA T,HATTORI S,SANO R,et al.Arabidopsis TRANSPARENT TESTA GLABRA2 is directly regulated by R2R3 MYB transcription factors and is involved in regulation of GLABRA2 transcription in epidermal differentiation[J].Plant Cell,2007,19(8):2531-2543.
- [53]MCGRATH K C,DOMBRECHT B,MANNERS J M,et al.Repressor-and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression[J].Plant Physiology,2005,139(2):949-959.
- [54]MAO P,DUAN M R,WEI C H,et al.WRKY62 transcription factor acts down stream of cytosolic NPR1 and negatively regulates jasmonate-responsive gene expression[J].Plant Cell Physiology,2007,48(6):833-842.
- [55]QIU D Y,XIAO J,DING X H,et al.OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate-and jasmonate-dependent signaling[J].Molecular Plant-microbe Interactions,2007,20(5):492-499.
- [56]QIU Y,JING S,FU J,et al.Cloning and analysis of expression profile of13WRKY genes in rice[J].Chinese Science Bulletin,2004,49(20):2159-2168.
- [57]JIANG Y,DEYHOLOS M K.Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes[J].BMC Plant Biology,2006,6(1):25.
- [58]KAWAURA K,MOCHIDA K,OGIHARA Y.Genome-wide analysis for identification of salt-responsive genes in common wheat[J].Functional&Integrative Genomics,2008,8(3):277-286.
- [59]蒋阿维,张素维,孙杨吾,等.珠美海棠MzWRKY基因家族盐胁迫应答模式研究[J].园艺学报,2010,37(8):1213-1219.
- [60]罗昌国,渠慎春,张计育,等.湖北海棠MhWRKY40b在几种胁迫下的表达分析[J].园艺学报,2013,40(1):1-9.