田玉富;崔璨;谢龙飞;李桂英;杨成君;

• 1:东北林业大学林学院

摘要(Abstract)：

从吉尔吉斯白桦转录组文库测序中获得白桦BkWRKY1转录因子的cDNA序列,该序列包含2 189个碱基。序列比对和同源性分析表明,该基因cDNA序列包含1 728 bp的开放阅读框,可编码575个氨基酸,5'非翻译区(UTR)为119 bp,3'非翻译区(UTR)为342bp。该基因属于WRKY transcription factor家族,含有2个WRKY结构域,蛋白分子量为62.483 kD,理论等电点为7.32,负电荷残基(Asp+Glu)总数为62个,正电荷残基(Arg+Lys)总数为62个。蛋白不含有信号肽,具有一定的亲水性,为亲水蛋白,无跨膜结构。同源性比较与进化树分析表明,吉尔吉斯白桦WRKY蛋白与大豆和蒺藜苜蓿的WRKY蛋白在进化上关系较近。该基因在0.6%NaHCO3胁迫处理后表达量增加,为上调表达基因。

关键词(KeyWords)： 吉尔吉斯白桦;WRKY转录因子;生物信息学分析

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项资金项目(DL12CA13);; 东北林业大学大学生创新训练项目(201310225101)

作者(Author): 田玉富;崔璨;谢龙飞;李桂英;杨成君;

Email:

DOI: 10.13989/j.cnki.0517-6611.2014.23.006

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